Molecular epidemiology of an acute gastroenteritis outbreak caused by GⅠ.6 Sapovirus in Taizhou, 2024
10.3760/cma.j.cn12866-20240903-00132
- VernacularTitle:2024年泰州市一起GⅠ.6型札如病毒急性胃肠炎暴发疫情的分子流行病学特征
- Author:
Jie ZHA
1
;
Yanqiu CAI
;
Jiang LI
;
Wenjun DAI
;
Da WANG
Author Information
1. 江苏省泰州市疾病预防控制中心微生物检验科，泰州 225300
- Publication Type:Journal Article
- Keywords:
Gastroenteritis outbreak;
Sapovirus;
GⅠ.6 genotype;
Molecular epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(3):333-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the pathogen of an acute gastroenteritis outbreak in a school in Taizhou, and to understand the epidemiological characteristics and patterns of the outbreak and the etiology of the pathogen.Methods:Twelve anal swab samples from patients who met the suspected case definition during the outbreak in March 2024 were collected. FilmArray GI was used to screen 22 common pathogens in the 12 samples, and the real-time fluorescent RT-PCR was performed for verification. Nested RT-PCR was used to amplify and sequence the nucleotide sequence of the viral capsid region VP1, and the variability of the sequence sites was analyzed. Based on the sequence relationship, a molecular phylogenetic tree was constructed and the viral genotypes were determined. Molecular transmission network analysis was conducted by integrating field epidemiological information.Results:The VP1 gene sequences were obtained from 8 of the 12 specimens. Systematic evolution showed that the molecular typing of the 8 strains of Sapoviuses (SaV) was all GI.6 genotype, and there was a C/T mutation at position 1236 in the VP1 gene. The inferred molecular transmission network was largely consistent with the information from the field epidemiological investigation, such as the initial infected person being the earliest case of the outbreak, and there was a closer transmission link between the two teachers.Conclusions:This article reported a school outbreak caused by a SaV GI.6 type. We speculated a more reliable molecular transmission network, estimated the rate of gene mutation and provided a reasonable elaboration for the C/T mutation at position 1236 of the VP1 gene.
