Research on the efficacy of terminal disinfection following spills of infectious materials in BSL-3 laboratories
10.3760/cma.j.cn112866-20241231-00190
- VernacularTitle:BSL-3实验室感染性物质溢洒后的终末消毒效果研究
- Author:
Wei ZHEN
1
;
Kun CAI
1
;
Weimin ZHOU
1
;
Guizhen WU
1
Author Information
1. 中国疾病预防控制中心病毒病预防控制所，北京 102206
- Publication Type:Journal Article
- Keywords:
Biosafety Level 3 laboratory;
Vaporized hydrogen peroxide;
Biological indicator;
Infectious materials
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(2):250-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the impact of vaporized hydrogen peroxide (VHP) on the terminal decontamination effectiveness following the spillage of infectious materials in a BSL-3 laboratory.Methods:Within BSL-3 laboratories with volumes of 92.5 m 3 and 218 m 3, two types of Geobacillus stearothermophilus spore strips were used as biological indicators. ATCC7953 was affixed to stainless steel strips, while ATCC12980 was applied to paper strips. The strips were soaked in cell culture medium and chicken embryo egg fluid to simulate minor spill models. While strips continuously immersed in cell culture medium or chicken embryo egg fluid served as major spill models. These were compared against untreated biological indicator strips to evaluate the terminal decontamination efficacy of VHP. Results:After the completion of terminal disinfection, in laboratories of different volumes and at various sampling points, the culture result of both types of bacterial strips from the minor spill models and the untreated control strips were all negative. In the major spill models, the positivity rate of the two types of bacterial strips soaked in cell culture medium was 75%, while the positivity rate of the two types of bacterial strips soaked in chicken embryo egg fluid was 100%.Conclusions:In the event of a large spill of infectious materials in a laboratory, to ensure biosafety, it is recommended to first treat the spilled materials before initiating the terminal disinfection process. Additionally, under the same disinfection conditions, the material of the biological indicator strips does not affect the terminal disinfection outcomes.
