Occult hepatitis B infection status and amino acid variation characteristics of HBcAb and S region among qualified blood donors in Jinhua area
10.3760/cma.j.cn112866-20240221-00021
- VernacularTitle:金华地区初筛合格献血者OBI感染现状及HBcAb与S区氨基酸变异特点
- Author:
Jiang QIAN
1
;
Xiaoming DU
1
;
Jun CAI
1
;
Xin WU
1
Author Information
1. 金华市中心血站，金华 321000
- Publication Type:Journal Article
- Keywords:
Occult hepatitis B virus infection;
HBV S;
Genotype;
Mutation site
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(3):340-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the status quo of occult hepatitis B virus infection (OBI) among blood donors in certain region and to explore the characteristics of amino acid variation between antibody to hepatitis B core antigen (HBcAb) and S region.Methods:The qualified blood samples from January 2019 to December 2022 were collected, and the OBI samples were screened by ELISA and HBV DNA. The OBI samples were quantitatively detected by HBcAb and HBV DNA and analyzed by HBV S region sequencing.Results:A total of 126 OBI positive samples were detected out of 188933 samples, the detection rate was 0.07%, and there was no significant difference in the detection rate of OBI between gender and blood donation times ( P>0.05). The highest OBI detection rate was found in 46-55 years old group (0.15%), other education group (0.35%) and occupational workers group (0.13%). The total detection rate of HBcAb (+ ) in 126 OBI samples was 90.48%. As the S/CO value of HBcAb (+ ) increased, the positive detection rate of HBV DNA also increased, and the correlation coefficient between HBcAb (+ ) and the quantitative value of HBV DNA was 0.782. The proportion of HBV B genotype in OBI blood donors was the highest (73.08%). A total of 15 OBI-related mutation sites were found, and there was no statistically significant difference among the mutation sites. Conclusions:OBI exists in blood donors in certain region, and HBcAb (+ ) is moderately positively correlated with the quantitative value of HBV DNA. HBV infection in OBI blood donors is mainly type B.
