Expression and generation of the polyclonal antibody of human group H rotavirus VP7 protein
10.3760/cma.j.cn112866-20240829-00125
- VernacularTitle:人H组轮状病毒VP7蛋白表达及其多克隆抗体制备
- Author:
Xiaoyu LIU
1
;
Ying ZHAO
;
Luqi ZHANG
;
Xuanze OUYANG
;
Qing ZHANG
;
Dandi LI
;
Jinsong LI
;
Lili LI
;
Xiaoman SUN
;
Zhaojun DUAN
Author Information
1. 甘肃中医药大学公共卫生学院，兰州 730000
- Publication Type:Journal Article
- Keywords:
Human group H rotavirus(RVH);
Baculovirus expression system;
VP7 protein;
Polyclonal antibody
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(6):702-708
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To express and block the VP7 protein of human group H rotavirus (group H rotavirus, RVH) J19, and to prepare the polyclonal adsorbent polyantibody function of VP7 for identification.Methods:The VP7 protein of human H rotavirus J19 strain was expressed by baculovirus system, and the protein was blocked by affinity chromatography and hydrophobic chromatography. The VP7 polyantiserum was prepared by immunizing the VP7 protein of human H group H rotavirus J19, and the VP7 polyantiserum was identified by ELISA, Western blot (WB) and immunofluorescence.Results:Soluble J19 VP7 protein was obtained. J19 VP7 rabbit polyclonal antibody was prepared. ELISA showed that VP7 polyclonal antibody had a good binding effect, binding to human RVH VP7 protein at 2 048 000-fold. WB showed that the polyclonal antibody could bind to J19 VP7 protein; Immunofluorescence assay showed that VP7 polyclonal antibody could specifically recognize human group H rotavirus J19.Conclusions:The J19 VP7 protein of human H rotavirus was successfully obtained, and the prepared VP7 polyclonal antibody could recognize human H rotavirus, which provided a basis for the detection of human H rotavirus and the study of the structure and function of the viral capsid.
