Evaluation of the preservation effects of 7 non-inactivating virus preservation solutions on H1N1 virus
- VernacularTitle:7种非灭活型病毒保存液对甲型H1N1流感病毒的保存效果评价
- Author:
Qun GAO
1
;
Dan WU
1
;
Jiachen ZHAO
1
;
Li ZHANG
1
;
Yu WANG
1
;
Yimeng LIU
1
;
Guilan LU
1
;
Xiaomin PENG
1
;
Wei DUAN
1
;
Daitao ZHANG
1
;
Quanyi WANG
1
;
Weixian SHI
1
Author Information
- Publication Type:Journal Article
- Keywords: Non-inactivating; Viral preservation solution; H1N1; HA titer; Evaluation of the preservation effects
- From: Chinese Journal of Experimental and Clinical Virology 2025;39(3):383-387
- CountryChina
- Language:Chinese
- Abstract: Objective:To evaluate the preservation efficacy of 7 non-inactivating virus preservation solutions.Methods:Equal amounts of H1N1 virus were added to 7 commercially available non-inactivating virus preservation solutions, and the samples were stored at -20 ℃, 4 ℃, 25 ℃ and 37 ℃ for 1 hour, 6 hours, 1 day, 3 days, and 5 days. The viral nucleic acid in each simulated sample under different storage conditions was measured using real-time quantitative PCR. The hemagglutination (HA) titer was determined through viral isolation culture and hemagglutination assay, comparing the differences in viral growth activity across different storage solutions and conditions.Results:Except for solution E, the other solutions effectively protected viral nucleic acid at the 4 storage temperatures. In terms of viral activity, solutions A, B, C, and D effectively maintained viral viability. A and B showing the best performance, E and F showed poorer performance, and G performed the worst.Conclusions:Most non-inactivating virus preservation solutions effectively protect viral nucleic acid, but there are significant differences in their ability to maintain viral viability. To ensure optimal virus preservation, it is recommended that medical institutions evaluate the effectiveness of preservation solutions before use.
