Inhibitory effect of nuclear transcription factor Nrf2 overexpression on HPV16 E6 expression in HEK293T
10.3760/cma.j.cn112866-202501-00000
- VernacularTitle:核转录因子Nrf2过表达对人乳头瘤病毒16型E6蛋白的抑制作用研究
- Author:
Jiao WANG
1
;
Hui WANG
1
;
Jieqiong ZHANG
1
;
Mingming WANG
1
;
Xuexin LU
1
;
Shiwen WANG
1
;
Ying LI
1
Author Information
1. 中国疾病预防控制中心病毒病预防控制所肿瘤病毒室 传染病溯源预警与智能决策全国重点实验室，北京 102206
- Publication Type:Journal Article
- Keywords:
Nuclear transcription factor-E2-related factor 2;
Cervical cancer;
Human papillomavirus type 16;
E6
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(2):169-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of nuclear factor erythroid-2 related factor (Nrf2) overexpression on high-risk human papillomavirus type 16 (HPV16) E6 protein.Methods:The pRK5-FLAG-NFE2L2 plasmid was constructed, and pRK5-FLAG-NFE2L2 and pRK5-HA-HPV16E6 plasmids were co-transfected in HEK293T cells, and the expression of the two proteins was detected by Western blotting (WB). Next, pRK5-HA-HPV16E6 and pRK5-FLAG-Nrf2 plasmids were expressed using an in vitro transcription system to observe the expression of both. Finally, pEGFP-HPV16E6 and pLVX-mCherry-Nrf2 plasmids were co-transfected in HEK293T cells, and the cellular localization of the E6 protein and the Nrf2 protein was observed using fluorescence microscopy.Results:Nrf2 protein was successfully overexpressed in HEK293T cells, and the WB result showed that Nrf2 inhibited HPV16 E6 protein expression in a significant dose-dependent manner. The expression of HPV16 E6 protein in the TNT Quick Coupled Transcription/Translation Systems was affected by Nrf2, while the expression of HPV16 E6 in TnT SP6 High-Yield Wheat Germ Protein Expression System was no longer inhibited by Nrf2. Fluorescence imaging further showed no intracellular co-localization of Nrf2 and HPV16 E6.Conclusions:Overexpression of the nuclear transcription factor Nrf2 reduces HPV16 E6 protein expression, but there is no intracellular co-localization of them.
