Genomic characterization and evolutionary analysis of hepatitis B virus subgenotype D3 in China in 2020
10.3760/cma.j.cn112866-20241106-00160
- VernacularTitle:2020年我国D3亚型乙肝病毒的基因组特征和进化分析
- Author:
Hui XIANG
1
;
Shuang ZHANG
;
Feng WANG
;
Feng QIU
;
Fuzhen WANG
;
Liping SHEN
;
Qiudong SU
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 国家卫生健康委医学病毒和病毒病重点实验室，北京 102206
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Subgenotype D3;
Molecular epidemiology;
Amino acid mutation;
Evolutionary rate
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(1):62-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the genetic characteristics and evolutionary origin of hepatitis B virus (HBV) subgenotype D3 in China in 2020.Methods:Serum samples and demographic details from patients infected with HBV D3 subgenotype were collected. HBV genomic sequences were obtained by nested PCR amplification and subsequent sequencing. Phylogenetic analysis, nucleotide homology, amino acid mutation and evolution rate of the S protein were conducted by comparing with reference sequence using bioinformatics tools.Results:The complete HBV gene sequences of 14 samples of D3 subtype HBV were obtained. Compared with 97 reference sequences, it was found that the sequences with the highest homology were from India, Mongolia, Iran and China, with the homology ranging from 96.0% to 97.9%. Mutations of 24 amino acids were found in 14 strains of D3 subtype. Among them, T131A, Y134F and T140I were associated with immune escape-related mutations. The genetic diversity of HBV D3 subtype increased slowly before 1975, remained relatively constant from 1975 to 2000, and began to decline after 2000. Evolutionary rate analysis showed that samples QGLD D3-02 and 03 originated from a common ancestor with the Iranian reference strain in 1872, and the other 12 samples QGLD D3-04-17 originated from a common ancestor with the Mongolian reference strain in 1843.Conclusions:The gene sequence of HBV D3 subtype in China had the highest homology with reference sequences from India, Iran, Mongolia and China. Evolutionary rate analysis revealed that 14 cases of HBV D3 subtype originated from a common ancestor with reference strains from Mongolia and Iran, which enriched the sequence and evolution information of HBV D3 subtype and provided a reference basis for the molecular epidemiological study of HBV.
