Investigation of hantavirus carriage in rodents and whole-genome sequence analysis in Shandong province, 2022
10.3760/cma.j.cn112866-20241217-00179
- VernacularTitle:2022年山东省鼠类汉坦病毒携带情况调查及其全基因组序列分析
- Author:
Yuwei LIU
1
;
Mingxiao YAO
;
Jinyan ZHANG
;
Qing DUAN
;
Bo PANG
;
Wenji ZHAI
;
Renpeng LI
;
Zengqiang KOU
Author Information
1. 山东第二医科大学公共卫生学院，潍坊 261053
- Publication Type:Journal Article
- Keywords:
Hantavirus;
Rodents;
Whole genome
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(1):56-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the situation of rodents carrying Hantavirus and the genetic and evolutionary characteristics of the virus in Zibo city, Shandong province in 2022, and provide reference for the scientific prevention and control of hemorrhagic fever with renal syndrome (HFRS).Methods:Real-time quantitative PCR (RT-qPCR) was used to detect hantavirus (HV) nucleic acids in rodent lung tissues and identify HV genotypes. Each nucleic acid fragment was designed to amplify various gene fragments by segment, and the whole genome of Hantavirus was sequenced by second generation sequencing. Sequence assembly was performed using SeqMan 7.1.0.44, a subprogram of DNAStar. Sequence alignment and evolutionary analysis were conducted using MEGA 7.0 and BioEdit software.Results:A total of 270 host animals were captured in this survey. Among them, 13 rodent lung samples tested positive for Hantavirus, resulting in a virus-positive rate of 4.8%. The full-genome sequences of four hantavirus strains were successfully obtained, all identified as Seoul virus (SEOV) genotype. Four Hantavirus-positive samples showed high nucleotide sequence homology in the M gene and belonged to the SEOV S3 subtype. These strains exhibited high similarity with those from Hebei, Liaoning, and Beijing. The amino acid sequences of the nucleoprotein and glycoprotein immunogenic epitopes were identical to those of the vaccine strain Z37.Conclusions:This study successfully determined the full genome sequences of four hantavirus strains from Zibo city, Shandong province. The genotypes are primarily SEOV, with the subtype being S3. The homology of genes within the same subtype is high, with no significant variations observed. The alignment of immune epitopes in key proteins suggests that the current vaccine may provide protection against locally circulating strains, but further in-depth research is still required.
