Molecular epidemiological characteristics of GII.3P12 norovirus outbreaks in China, 2022-2023
10.3760/cma.j.cn112866-20241219-00182
- VernacularTitle:2022—2023年我国GII.3[P12]诺如病毒暴发分子流行病学特征
- Author:
Lijiao CAO
1
;
Yanhui YANG
1
;
Shuting YANG
1
;
Qing ZHANG
1
;
Xiangyu KONG
1
;
Hong WANG
1
;
Miao JIN
1
;
Zhaojun DUAN
1
Author Information
1. 中国疾病预防控制中心病毒病预防控制所 传染病溯源预警与智能决策全国重点实验室 卫生部医学病毒和病毒病重点实验室，北京 102206
- Publication Type:Journal Article
- Keywords:
Norovirus;
Genotype;
Molecular evolution;
Epitope prediction
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(1):27-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the molecular evolutionary characteristics of the recombinant strain GII.3[P12] of norovirus acute gastroenteritis outbreaks and aggregated outbreaks in China from 2022 to 2023.Methods:Epidemiological information, case information, clinical samples, as well as detection and genotyping information of norovirus outbreaks and aggregated outbreaks from 2022 to 2023 were collected; positive samples of the GII.3[P12] recombinant strain were subjected to nucleic acid extraction, whole-genome amplification, and sequence analysis; and homology simulation method were used to construct a three-dimensional structure and predict antigenic epitopes.Results:From January 2022 to December 2023, a total of 1 136 norovirus outbreaks and aggregated outbreaks were reported in China′s norovirus outbreak surveillance network, and genotyping result were successfully obtained for 942 outbreaks, with GII dominating, accounting for 76.0% (716/942), and the proportion of GI and mixed genotypes being 15.8% (149/942) and 8.2% (77/942). Norovirus outbreaks caused by GII were dominated by GII.3[P12] (22.5%, 161/716), while other major genotypes included GII.17[P17] (18.7%, 134/716), GII.4_Sydney 2012[P16] (11.6%, 83/716) and GII.6[P7] ( 11.3%, 81/716). 2022-2023 Outbreaks caused by GII.3[P12] were concentrated in February-March (54.0%, 87/161), with the main outbreaks occurring in nursery and primary schools (87.5%), the mode of transmission was mainly human-to-human (68.9%), and the main susceptible population was children aged 3-7 years (93.3%). In this study, the genome sequences of 25 GII.3[P12] recombinant strains were obtained, and according to the phylogenetic analysis, it was shown that the GII.3[P12] recombinant strains in China in 2022-2023 belonged to the Cluster IV cluster of sublineage b (7 strains) and sublineage c (18 strains). A total of 11 linear and 8 conformational epitopes were predicted by epitope prediction analysis, and the predicted linear and conformational epitopes had overlapping positions, and each conformational epitope was part of the predicted linear epitope with conserved potential antigen-binding and receptor-binding sites.Conclusions:The recombinant strain GII.3[P12] is one of the epidemic strains that will cause outbreaks and clusters of norovirus in China in 2022-2023, and its genome did not undergo significant mutation.
