Complete genome sequence analysis of imported dengue type 1 virus in Gansu province in 2023
10.3760/cma.j.cn112866-20240825-00123
- VernacularTitle:甘肃省2023年输入性登革1型病毒全基因组序列分析
- Author:
Haizhuo WU
1
;
Shu LIANG
1
;
Maoxing DONG
1
;
Zhongyi JIANG
1
;
Huan WEI
1
;
Xiaoshu ZHANG
1
Author Information
1. 甘肃省疾病预防控制中心病毒实验室 甘肃省传染病病原学重点实验室（培育基地），兰州 730000
- Publication Type:Journal Article
- Keywords:
Dengue fever;
Dengue virus type 1;
Whole genome sequencing;
Amino acid site analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(6):655-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To conduct whole-genome sequencing of two serum samples from imported dengue fever (DF) cases in Gansu province in 2023, analyze the molecular biological characteristics of the dengue virus (DENV) strains, accumulate baseline data for DF research in Gansu province, and provide a scientific basis for the prevention and control of imported epidemics.Methods:Two serum samples that tested positive for DENV nucleic acid were sequenced for their entire genome using a third-generation sequencing platform. The obtained sequences were subjected to phylogenetic analysis, sequence homology analysis, amino acid sequence similarity analysis, and analysis of differences in mutation sites.Results:Two complete DENV genomes were obtained through third-generation sequencing, with sequence lengths of 10 715 bp and 10 714 bp respectively. The nucleotide similarity between them was 99.11%, and the amino acid similarity was 99.59%. Homology and phylogenetic analyses indicated that both sequences belonged to DENV type 1 genotype Ⅲ and were highly homologous to isolates from Singapore, India, Nepal, and the Guangzhou city of China. The nucleotide similarity is greater than 96.91%, and the amino acid similarity is greater than 98.70%. Analysis of amino acid site differences showed that DENV-2023-GS001 and DENV-2023-GS002 had 10 and 14 mutation sites in the E protein, respectively, but the main sites related to virulence remained unchanged.Conclusions:In 2023, two cases of imported DF in Gansu province were identified as genotype Ⅲ of DENV type 1. In terms of genetic evolutionary relationships, these viruses are most closely related to those from countries such as Singapore, India, and Nepal. This indicates that there remains a risk of DENV importation and transmission in Gansu province.
