Study of correlation between C1GalT1 protein and disease activity in ulcerative colitis
10.3760/cma.j.cn101480-20230914-00019
- VernacularTitle:肠上皮C1GalT1蛋白与溃疡性结肠炎疾病活动度的相关性研究
- Author:
Zehui WANG
1
;
Wei JIANG
1
;
Yihan LU
1
;
Dan LI
1
;
Hang ZHANG
1
;
Jun ZHANG
1
Author Information
1. 南京医科大学附属南京医院消化内科，南京 210006
- Publication Type:Journal Article
- Keywords:
Ulcerative colitis;
Core 1 β1, 3-galactosyltransferase;
Disease activity;
O-glycans;
Correlation
- From:
Chinese Journal of Inflammatory Bowel Diseases
2024;08(6):434-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the relationship between the expression level of core 1 β1, 3-galactosyltransferase (C1GalT1) and activity of ulcerative colitis (UC), and to explore its potential use in predicting UC activity.Methods:A retrospective case-control study was constructed. The data of 77 UC patients (UC group) and 18 healthy controls (normal contro group) were collected from November 2013 to February 2023 at the Department of Gastroenterology, Nanjing Hospital of Nanjing Medical University. Immunohistochemistry was used to measure the expression of C1GalT1 protein in the intestinal epithelium of both groups. The modified Mayo score was used to assess UC disease activity, with scores ≤ 2 and individual items < 2 considered as remission, scores of 3-5 as mild activity, scores of 6-10 as moderate activity, and scores ≥ 11 as severe activity. Statistical analysis was conducted to compare the differences in C1GalT1 protein expression between the UC group and the normal control group, as well as among UC patients with different activities. The Spearman test was used to analyze the correlations of C1GalT1 protein with disease activity, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR). The diagnostic efficacy of C1GalT1 protein for UC activity was evaluated using receiver operating characteristic (ROC) curves.Results:In the UC group, there were 33 males and 44 females, with an average age of (48.22 ± 1.81) years and a disease duration of 4.0 (0.5, 10.0) years. The group included 9 patients in remission, 15 with mild activity, 34 with moderate activity, and 19 with severe activity. In the normal control group, there were 7 males and 11 females, with an average age of (53.44 ± 3.35) years. There were no statistically significant differences in age and gender between the two groups (both P > 0.05). The expression of C1GalT1 protein in UC patients was lower than that in the normal control group ( P < 0.000 1). Additionally, as UC activity increased, C1GalT1 protein expression gradually decreased, with all differences being statistically significant (all P < 0.05). C1GalT1 protein was negatively correlated with UC activity ( r = -0.716 1, P < 0.000 1), CRP ( r = -0.334, P < 0.01), and ESR ( r = -0.383, P < 0.001). Regarding the diagnostic efficacy of distinguishing between the normal control and UC groups, when the cutoff value for C1GalT1 protein was set at 33.16%, the sensitivity was 94.4%, the specificity was 98.7%, and the area under curve (AUC) was 0.994. For diagnosing mild to moderate activity, when the cutoff value was 29.96%, the sensitivity was 100%, the specificity was 91.2%, and the AUC was 0.978. For diagnosing moderate to severe activity, when the cutoff value was 26.9%, the sensitivity was 79.4%, the specificity was 78.9%, and the AUC was 0.805. Conclusion:The expression of glycosyltransferase C1GalT1 protein is significantly correlated with the activity of UC patients and may serve as a marker for UC activity.
