Study on the protective effect of renal interstitial fibrosis by 1,25(OH)2D3 based on NF-κB signaling pathway
10.3969/j.issn.1673-9701.2025.25.014
- VernacularTitle:基于NF-κB信号通路探讨1,25(OH)2D3保护肾间质纤维化的研究
- Author:
Huifeng GONG
1
;
Yijing KONG
;
Chunjiang ZHANG
;
Dong LIU
;
Zhifeng LIN
Author Information
1. 石河子大学第一附属医院肾内科,新疆石河子 832008
- Publication Type:Journal Article
- Keywords:
Nuclear factor κB signaling pathway;
1,25-dihydroxy vitamin D3;
Renal interstitial fibrosis;
Inflammatory cytokine
- From:
China Modern Doctor
2025;63(25):61-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of 1,25-dihydroxy vitamin D3[1,25(OH)2D3]in renal interstitial fibrosis mediated by nuclear factor κB(NF-κB)and inflammatory cytokines.Methods Taking the renal interstitial fibrosis model induced by transforming growth factor-β1(TGF-β1)as the research object,they were divided into blank group(HK-2 cells+complete culture medium),model group(5ng/ml TGF-β1 stimulated HK-2 cells for 48 hours),intervention group A[with 10-7mol/L 1,25(OH)2D3 intervention for 24 hours on the basis of model group],intervention group B[with 10-7mol/L 1,25(OH)2D3 intervention for 48 hours on the basis of model group]and intervention group C[with 10-7mol/L 1,25(OH)2D3 intervention for 72 hours on the basis of model group].The cell morphology,activity,protein expression and inflammatory factor levels of each group were observed and compared.Results The cell viability of model group was significantly lower than that of blank group(P＜0.05),the cell viability of intervention groups A,B and C was significantly higher than that of model group(P＜0.05).The protein expressions of p-NF-κBp65/NF-κBp65 and smooth muscle actin α(α-SMA),as well as the levels of interleukin-6(IL-6)and tumor necrosis factor α(TNF-α)in model group were significantly higher than those in blank group,while the protein expression of E-cadherin was significantly lower than that in blank group(P＜0.05).The protein expressions of p-NF-κBp65/NF-κBp65 and α-SMA,as well as the levels of IL-6 and TNF-α in intervention groups A,B and C were significantly lower than those in model group,while protein expression of E-cadherin was significantly higher than that in model group(P＜0.05).Among them,the change in intervention group A was the most significant.Conclusion 1,25(OH)2D3 can alleviate renal interstitial fibrosis by regulating the NF-κB signaling pathway and inflammatory cytokines,and 24 hours may be the optimal intervention time window.
