Establishment of an in situ tumor model of U87MG-Luc glioblastoma in nude mice
10.3969/j.issn.1673-9701.2025.25.004
- VernacularTitle:U87MG-Luc裸鼠胶质母细胞瘤原位模型建立
- Author:
Yazhou WANG
1
;
Jianfeng HU
;
Yijia LIU
;
Yuxuan TONG
Author Information
1. 柳州市工人医院GCP办公室,广西柳州 545000
- Publication Type:Journal Article
- Keywords:
Glioblastoma;
Animal model;
Temozolomide;
Nude mice
- From:
China Modern Doctor
2025;63(25):13-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a stable cell line expressing luciferase using U87MG cells,and to establish a nude mouse glioblastoma in situ transplantation tumor model,in order to provide a suitable efficacy evaluation model for anti-glioblastoma drugs.Methods A stable and highly expressing luciferase U87MG cell line(U87MG-Luc)was prepared by infecting U87MG with lentivirus,and its stability was verified by repeated passages.U87MG-Luc single-cell suspension,which has been amplified and digested in vitro,was injected into the caudate nucleus of the right brain of nude mice using a brain stereotaxic device and a micro constant flow pump.Using small animal live imaging technology to observe the tumor formation and growth of U87MG-Luc inoculated,and confirm appropriate modeling conditions.Subsequently,the U87MG-Luc brain in situ tumor model was constructed to validate the efficacy of the first-line clinical treatment drug temozolomide,including indicators such as tumor growth status,animal weight,and survival time.Results Successfully established and screened U87MG-Luc stable cell line using lentiviral infection system.The luciferase expressed by stable strains was linearly related to cell seeding density,and the in vitro culture could stably express luciferase after continuous passage to the 21st generation.After inoculating U87MG-Luc cells at an appropriate concentration into the brain of nude mice,in situ brain tumor formation was achieved,with a high success rate(100％)of 2 × 105 cells/nude mouse.On the fifth day after inoculation,the brain tumor remained stable with no metastasis,and the tumor growth trend was good thereafter.In the U87MG-Luc brain in situ tumor model constructed in this study,temozolomide significantly inhibited tumor growth and prolonged the survival of tumor bearing nude mice(P＜0.001).Conclusion A stable and highly expressing luciferase U87MG-Luc cell line was successfully constructed,transduced,and screened based on the lentiviral expression system,and U87MG-Luc nude mouse brain in situ tumor model was constructed to provide a suitable pharmacological evaluation model for the discovery of anti-glioma drugs.
