The role of cell-free DNA in inflammatory bowel disease
10.3760/cma.j.cn101480-20230118-00008
- VernacularTitle:胞外游离DNA在炎症性肠病中的作用研究
- Author:
Songjie WANG
1
;
Zhe ZHANG
1
;
Xia LIU
1
;
Dandan WANG
1
;
Yanfang LIU
1
;
Baisui FENG
1
Author Information
1. 郑州大学第二附属医院消化内科，郑州 450014
- Publication Type:Journal Article
- Keywords:
Inflammatory bowel disease;
Ulcerative colitis;
Crohn′s disease;
Cell-free DNA;
Disease severity
- From:
Chinese Journal of Inflammatory Bowel Diseases
2023;07(4):331-334
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differences in cell-free DNA (cfDNA) concentrations between the inflammatory bowel disease (IBD) patients with different disease severities and the significance.Methods:Cross-sectional study was conducted. Ninety-one patients with IBD were included in the Second Affiliated Hospital of Zhengzhou Univesity from April 2021 to May 2022, including 48 patients with ulcerative colitis (UC) and 43 patients with Crohn′s disease (CD). Fifteen healthy people were assigned as healthy control group. The differences in cfDNA concentration between IBD group and healthy control group and between IBD patients with different disease severities were compared. The correlations between cfDNA concentration and laboratory indicators of IBD patients were analyzed.Results:The cfDNA concentration of IBD patients was higher than that of healthy control group [ (86.7±22.6) ng/ml vs. (52.6±15.8) ng/ml, P<0.001], and the cfDNA concentrations of UC and CD patients were higher than that of healthy control group (both P<0.001), the differences were statistically significant. The cfDNA concentration of mild, moderate and severe UC patients increased successively, and the differences were statistically significant [ (62.9±11.1) ng/ml vs. (83.6±17.1) ng/ml vs. (103.9±19.1) ng/ml, all P<0.01]. cfDNA concentration of UC patients was positively correlated with disease activity ( r = 0.74, P<0.001). cfDNA concentration of active CD patients was significantly higher than that in remission stage [ (104.2±17.2) ng/ml vs. (78.6±18.6) ng/ml, P<0.001]. cfDNA concentration in CD patients was positively correlated with disease activity ( r = 0.58, P<0.001). cfDNA concentration in UC patients was positively correlated with C-reactive protein (CRP) ( r = 0.4, P<0.01) and erythrocyte sedimentation rate (ESR) ( r = 0.37, P<0.01), and negatively correlated with hemoglobin ( r = -0.46, P<0.01) and albumin ( r = -0.51, P<0.01). cfDNA concentration in CD patients was positively correlated with CRP ( r = 0.48, P<0.01) and ESR ( r = 0.38, P<0.05), and negatively correlated with hemoglobin ( r = -0.45, P<0.01) and albumin ( r = -0.32, P<0.05) . Conclusion:cfDNA concentration closely correlates with disease severity of IBD patients and is a new indicator to evaluate disease severity of IBD patients.
