Research on brown adipose tissue-derived exosomes regulating Pink1-Parkin pathway-mediated mitophagy to ameliorate endothelial cell injury
10.3760/cma.j.cn311282-20250324-00158
- VernacularTitle:棕色脂肪组织外泌体调控Pink1-Parkin通路介导线粒体自噬改善内皮细胞损伤的研究
- Author:
Xiaoyong HU
1
;
Zhaoying YANG
1
;
Qianhua SONG
1
;
Ailijiang ZUKELAI
1
;
Rui TANG
1
;
Huan WANG
1
;
Hongjian LI
1
Author Information
1. 新疆医科大学第五临床医学院高血压科，乌鲁木齐 830000
- Publication Type:Journal Article
- Keywords:
Cardiovascular diseases;
Cell injury;
Brown adipose tissue-derived exosomes;
Mitophagy;
Pink1-Parkin pathway
- From:
Chinese Journal of Endocrinology and Metabolism
2025;41(8):672-680
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether brown adipose tissue-derived exosomes(BAT-exos) could ameliorate endothelial cell injury by activating Pink1-Parkin pathway-mediated mitophagy.Methods:Endothelial cell injury was induced with angiotensin Ⅱ(Ang Ⅱ) to establish a cellular injury model. Exosomes were isolated from both brown adipose tissue and white adipose tissue and characterized by transmission electron microscopy(TEM), nanoparticle tracking analysis(NTA), fluorescence labeling, and Western blot. Cell viability was assessed using the CCK-8 assay, and apoptosis rates were determined by flow cytometry. Levels of tumor necrosis factor-α(TNF-α), interleukin(IL)-6, and IL-8 were measured by ELISA. Mitochondrial autophagy was assessed by immunofluorescence colocalization, and protein expression levels of Pink1, Parkin, and LC3 Ⅱ/I were determined by Western blot.Results:Ang Ⅱ induced endothelial cell apoptosis, activated inflammatory responses, and suppressed mitophagy, as evidenced by decreased expression of mitophagy-related proteins. Following the successful characterization of BAT-exos, we found that BAT-exos activated mitophagy and alleviated endothelial cell injury, whereas white adipose tissue-derived exosomes(WAT-exos) inhibited mitophagy and exacerbated injury. Mechanistically, BAT-exos targeted the Pink1-Parkin signaling pathway to activate mitophagy.Conclusion:BAT-exos markedly improve endothelial cell injury by activating mitophagy through the Pink1-Parkin pathway, providing new insights and potential therapeutic targets for cardiovascular diseases.
