Expression and significance of glycoprotein nonmetastatic melanoma protein B in mice with chronic intestinal fibrosis
10.3760/cma.j.cn101480-20220411-00058
- VernacularTitle:糖蛋白非转移性黑色素瘤B在小鼠慢性肠道纤维化中的表达及意义
- Author:
Shumei BAO
1
;
Hui LI
1
;
Yajie ZHANG
1
;
Linyan ZHOU
1
;
Ying XIE
1
;
Feng TIAN
1
Author Information
1. 中国医科大学附属盛京医院消化内科，沈阳 110004
- Publication Type:Journal Article
- Keywords:
Inflammatory bowel disease;
Glycoprotein nonmetastatic melanoma protein B;
Dextran sulfate sodium;
Intestinal fibrosis;
Mice
- From:
Chinese Journal of Inflammatory Bowel Diseases
2022;06(4):335-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of glycoprotein nonmetastatic melanoma protein B (Gpnmb) in chronic intestinal fibrosis of mice induced by dextran sodium sulfate (DSS) .Methods:Twelve BALB/c mice were randomly and equally divided into model group and control group. The mice in model group received water containing 2.5% dextran sodium sulfate (DSS) to establish a chronic intestinal fibrosis model, while the mice in control group were not treated. The body mass, colon length, colonic histomorphology score and histological damage score of mice were calculated. The inflammatory degree of colitis was assessed by HE staining and the degree of colonic fibrosis was assessed by Masson staining. The protein expression of collagen typeⅠ alpha 2 (Col1α2) , Gpnmb and its receptor CD44 in colonic tissues was determined by immunohistochemistry and Western blot. The mRNA expression of Col1α2 and Gpnmb was detected by fluorescent quantitative PCR. The differences of the above indexes between the two groups were compared by t test. Results:Compared with the control group, the colon length of the model group was shorter and the colonic histomorphology score was higher (all P<0.05) . HE staining results showed that the intestinal glands in the colonic mucosa were disorderly arranged, atrophic and reduced, the goblet cells were less, and edema, neutrophil and lymphocyte infiltration were seen in the mucosa and submucosa in the model group. The mucosa of the control group was normal without inflammation. Compared with the control group, the histological damage score of the colon in the model group was higher and the fibrotic area was larger, the protein expression of Col1α2, Gpnmb and CD44 was higher, the mRNA expression of Col1α2 and Gpnmb were higher (all P<0.05) . Conclusion:Gpnmb may promote the occurrence and development of DSS-induced chronic intestinal fibrosis in mice through CD44.
