Analysis of gut microbiota in inflammatory bowel disease patients with Clostridium difficile infection
10.3760/cma.j.cn101480-20220324-00049
- VernacularTitle:炎症性肠病合并艰难梭菌感染患者的肠道菌群研究
- Author:
Si YU
1
;
Yue LI
1
;
Hui XU
1
;
Bowen TIAN
1
;
Yujie SHI
1
;
Jiaming QIAN
1
Author Information
1. 中国医学科学院 北京协和医学院 北京协和医院消化内科，北京 100730
- Publication Type:Journal Article
- Keywords:
Inflammatory bowel disease;
Clostridium difficile;
Gut microbiota;
Opportunistic infection
- From:
Chinese Journal of Inflammatory Bowel Diseases
2023;07(1):48-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the character of gut microbiota in inflammatory bowel disease (IBD) patients with Clostridium difficile infection (CDI) . Methods:A cross-sectional study was conducted. Fifty-four IBD patients were included consecutively in Peking Union Medical College Hospital from November 2017 to April 2018. The patients were divided into CDI group ( n = 20) and non-CDI group ( n = 34) according to whether the patient had CDI. Forty healthy people were recruited as the healthy control group. 16S rRNA sequencing was carried out in all the samples of 3 groups to analyze the diversity and composition of gut microbiota. Results:Compared with the healthy control group, α diversity (Chao1 index, Shannon index, Simpson index, Pielou index) was significantly lower and β diversity (microbiota structure) was significantly different in CDI and non-CDI groups (all P<0.05) . There was no significant difference in α and β diversities between CDI and non-CDI groups (all P>0.05) . Compared with healthy control group, relative abundance of Faecalibacterium was significantly lower in CDI and non-CDI groups, while relative abundances of Proteobacteria and Enterobacteriaceae were significantly higher (all P<0.05) . Compared with non-CDI group, relative abundances of Parabacteroides and Rothia were significantly lower, while relative abundance of Clostridioides was significantly higher in CDI group (all P<0.05) . Lefse analysis results showed that Ruminococcus gnavus, Clostridium innocuum, Clostridium paraputrificum, Lactobacillus farciminis, and Peptostreptococcaceae contributed greatly to the difference in CDI group. Conclusion:Compared with healthy control group and non-CDI group, IBD patients with CDI have specific alterations in composition of gut microbiota.
