Role of cancer-associated fibroblasts autophagy in papillary thyroid cancer
10.3760/cma.j.cn311282-20241006-00448
- VernacularTitle:癌相关成纤维细胞自噬对甲状腺乳头状癌影响的研究
- Author:
Xuemei ZHANG
1
;
Danyang SUN
;
Ning LI
;
Qicheng ZHANG
;
Ke XU
;
Wei ZHENG
;
Qiang JIA
;
Jian TAN
;
Zhaowei MENG
Author Information
1. 天津医科大学总医院核医学科，天津 300052
- Publication Type:Journal Article
- Keywords:
Cancer-associated fibroblasts;
Papillary thyroid cancer;
Tumor microenvironment;
Autophagy
- From:
Chinese Journal of Endocrinology and Metabolism
2025;41(2):135-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inpact of thyroid cancer-derived cancer-associated fibroblasts(CAF) autophagy on papillary thyroid cancer(PTC).Methods:CAF and normal fibroblasts were isolated from cancerous and adjacent normal thyroid tissues from four PTC patients. Expressions of fibroblast activation protein(FAP) and α-smooth muscle actin in cells were assessed. Conditioned medium of CAF and normal fibroblasts were prepared and used to culture PTC cells. The effects of CAF and normal fibroblasts on survival, proliferation, migration, invasion and iodine uptake of PTC cells were evaluated through cell proliferation assay, cell scratch assay, cell invasion assay, and cell iodine uptake assay. The autophagy level of CAF was also evaluated. Autophagy inhibition and activation were used to regulate the autophagy of CAF, and then their effects on PTC cell proliferation, migration and invasion were further evaluated. The in vivo effect of CAF autophagy on PTC xenograft tumor growth was evaluated.Results:CAF exhibited higher FAP expression and basal autophagy levels. PTC cells co-cultured with CAF-conditioned media showed enhanced proliferation, migration, invasion, and reduced iodine uptake. Autophagy inhibition reduced these effects, while autophagy activation further promoted them. In vivo, inhibiting CAF autophagy suppressed tumor growth.Conclusions:CAF promotes PTC cell malignancy through autophagy activation, enhancing proliferation, migration, and invasion while reducing iodine uptake.
