The effect and mechanism of Jianpi Qinghua granule in improving skeletal muscle insulin resistance induced by cadmium exposure in rats
10.3760/cma.j.cn311282-20240926-00437
- VernacularTitle:健脾清化颗粒改善镉暴露大鼠骨骼肌胰岛素抵抗的作用和机制
- Author:
Chi CHEN
1
;
Yuan CHEN
;
Ningjian WANG
;
Junfei XU
;
Xu HAN
;
Yanyan XIAO
;
Jing TIAN
;
Chao WANG
;
Mengjie CAI
;
Qingguang CHEN
;
Hao LU
Author Information
1. 上海中医药大学附属曙光医院内分泌科 201203
- Publication Type:Journal Article
- Keywords:
Diabetes;
Insulin resistance;
Cadmium;
Jianpi Qinghua granule;
Oxidative stress
- From:
Chinese Journal of Endocrinology and Metabolism
2025;41(1):54-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of Jianpi Qinghua granule in improving skeletal muscle insulin resistance induced by long-term low-dose cadmium exposure in rats.Methods:A total of 24 SPF-grade healthy 2-month-old Sprague-Dawley rats were assigned to normal control(NC) group, cadmium exposure(Cd) group, and Jianpi Qinghua granule protection(Cd+ JPQHG) group. After 24 weeks, fasting blood glucose and insulin levels were measured, and HOMA-IR was calculated. The intraperitoneal glucose tolerance test and insulin tolerance test were conducted to assess insulin sensitivity. Skeletal muscle tissues were extracted for Western blot analysis to detect levels of insulin signaling pathway-related proteins. Immunofluorescence was used to assess the translocation of glucose transporter 4(GLUT4), and oxidative stress markers were measured.Results:Compared to the NC group, the Cd group showed significant increases in fasting plasma glucose, fasting insulin levels, and HOMA-IR after 24-week exposure. Abnormal glucose and insulin tolerance were also observed in the Cd group. The 12-week intervention with Jianpi Qinghua granule significantly improved glucose metabolism and alleviated the abnormalities in glucose and insulin tolerance. Western blot results indicated that the phosphorylation levels of PI3K and Akt in the skeletal muscle of the Cd group were significantly reduced compared to the NC group, while these levels were significantly elevated in the Cd+ JPQHG group, along with increased translocation of GLUT4 to the cell membrane. Additionally, cadmium exposure significantly increased H 2O 2 and malondialdehyde levels while decreased antioxidant enzyme activity. These oxidative stress indicators improved significantly after Jianpi Qinghua granule intervention( P<0.05). Conclusion:Jianpi Qinghua granule may improve skeletal muscle insulin resistance and glucose metabolism disorders due to long-term low-dose cadmium exposure by reducing oxidative stress, regulating the phosphorylation levels of key proteins in the insulin signaling pathway, and promoting GLUT4 translocation to the cell membrane.
