Effects of different protein diets on mice with colitis
10.3760/cma.j.cn101480-20190522-00074
- VernacularTitle:不同蛋白种类的膳食对结肠炎小鼠的影响
- Author:
Miao FANG
1
;
Xiuwen WU
;
Tao ZHENG
;
Wenbin GONG
;
Jianan REN
Author Information
1. 南京医科大学金陵临床医学院，南京 210009
- Publication Type:Journal Article
- Keywords:
Protein;
Diet;
Colitis;
Enteral nutrition
- From:
Chinese Journal of Inflammatory Bowel Diseases
2021;05(3):225-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the effects of casein, whey and soy protein diet on treating colitis.Methods:Fifty C57BL/6 mice were randomly and equally divided into control group, general diet group, casein group, whey group and soy group with 10 mice in each group. Mice were fed with corresponding diets for 14 days. Acute colitis mice modelof general diet group, casein group, whey group and soy group were induced by dextran sulfate sodium (DSS) on the 7th day. The disease activity index (DAI) score was evaluated and recorded. The serum and colon tissues of each group on the 14th day were collected todetect the serum levels of IL-6, TNF-α and IL-10 by ELISA, and to calculate the pathological score of colon.Results:The DAI score of the whey group was significantly lower than that of the casein group and soy protein group (all P<0.05) , and higher than that of the control group ( P<0.05) . The pathological score of colon tissue of the control group was significantly lower as compared to other 4 groups (all P<0.05) , while above scoreof whey group was significantly lower than that of the casein group and soy protein group (all P<0.05) , but there was no significant difference between the whey group and general diet group. The levels of serum IL-6 and TNF-α in were significantly lower than those in the casein group and soy group (all P<0.05) , and serum IL-10 level in the whey group was significantly higher than that in general diet, casein and soy groups (all P<0.05) . The level of colon IL-10 in the whey group was significantly higher than that in the casein group and soy group (all P<0.05) . Conclusion:The efficacy of whey protein diet on treating acute colitis mice is better compared to general diet, casein and soy protein diet.
