Association of colonic vitamin D receptor expression with imbalance of Th17/Treg cells in patients with ulcerative colitis
10.3760/cma.j.cn101480-20200113-00003
- VernacularTitle:溃疡性结肠炎患者结肠维生素D受体表达与Th17/Treg细胞失衡的关系
- Author:
Shenglong XIA
1
;
Quanjia MIN
;
Xiaoxiao SHAO
;
Shuguang CAO
;
Daopo LIN
;
Limin WU
;
Dingyuan HU
;
Duoduo QUAN
;
Yi JIANG
Author Information
1. 温州医科大学附属第二医院消化内科，浙江温州 325000
- Publication Type:Journal Article
- Keywords:
Ulcerative colitis;
Vitamin D receptor;
Th17 cells;
Treg cells
- From:
Chinese Journal of Inflammatory Bowel Diseases
2021;05(1):62-67
- CountryChina
- Language:Chinese
-
Abstract:
Objects:To explore the association of colonic vitamin D receptor (VDR) expressionwith imbalance of Th17/regulatory T cells (Tregs) in patients with ulcerative colitis (UC) .Methods:The specimens of peripheral blood and colonic tissue were retrospectively collected from 30 UC patients and 15 controls (colonic polypadenoma) , respectively. The clinical data of all the participants were simultaneously recorded, including age, gender, smoking, body mass index, UC activity (assessed by Mayo scoring system) . The electrochemiluminescence method was conducted to measure the serum level of 25-hydroxyvitamin D [25 (OH) D]. Moreover, the immunohistochemical staining method was applied for examining the protein expression levels of VDR, Th17 and Treg cells related factors (RORγt, IL-17, Foxp3, IL-10, respectively) in the colonic tissues.Results:Compared with the controls, the expressions of RORγt, IL-17, Foxp3 and IL-10, together with the ratio of RORγt/Foxp3, were significantly higher in UC patients [0.32 (0.20, 0.46) ×10 -2 vs. 0.17 (0.11, 0.19) ×10 -2, 0.56 (0.30, 0.76) ×10 -2 vs. 0.19 (0.16, 0.26) ×10 -2, 0.26 (0.16, 0.33) ×10 -2 vs. 0.18 (0.12, 0.21) ×10 -2, 0.29 (0.19, 0.37) ×10 -2 vs. 0.06 (0.04, 0.09) ×10 -2, 1.14 (0.81, 1.86) vs. 0.86 (0.78, 0.92) , all P<0.05], meanwhile the expressions of RORγt, IL-17, Foxp3, IL-10, and the ratio of RORγt/Foxp3 were higher in patients with active UC than inactive UC [0.45 (0.31, 0.53) ×10 -2 vs. 0.16 (0.13, 0.20) ×10 -2, 0.73 (0.56, 0.78) ×10 -2 vs. 0.26 (0.21, 0.33) ×10 -2, 0.26 (0.24, 0.35) ×10 -2 vs. 0.17 (0.14, 0.26) ×10 -2, 0.35 (0.28, 0.38) ×10 -2 vs. 0.16 (0.11, 0.20) ×10 -2, 1.77 (0.90, 2.07) vs. 82.84 (64.12, 112.67) , all P<0.05]. The colonic VDR expression in UC patients was significantly lower than that in controls [0.66 (0.28, 19.15) ×10 -2 vs. 2.94 (2.61, 3.21) ×10 -2, P<0.001], meanwhile VDR expression was also shown to be lower in patients with active UC than that in those with inactive UC [0.37 (0.26, 0.69) ×10 -2 vs. 2.63 (1.78, 3.24) ×10 -2, P<0.001]. The subsequent linear regression analysis suggested that the ratio of RORγt/Foxp3 in colonic tissue of UC patients was independently and negatively associated to the colonic VDR expression ( β = -0.530, P = 0.003) . Conclusion:The lower colonic VDR expression is associated to the imbalance of Th17/Treg cells in UC patients.
