Mechanism study on genesis and development of enteritis induced by deoxycholic acid-mediating dysbosis of intestinal microbiota and bile acid abnormal metabolism
10.3760/cma.j.cn101480-20200624-00072
- VernacularTitle:脱氧胆酸介导的肠道菌群失衡和胆汁酸代谢异常促进肠炎发生发展的机制研究
- Author:
Mengsha CEN
1
;
Yubin ZHU
1
;
Yuqin SHEN
1
;
Fangli CHENG
1
;
Xia ZHENG
1
;
Weiling HU
1
;
Ning DAI
1
;
Mengque XU
1
Author Information
1. 浙江大学医学院附属邵逸夫医院消化内科，杭州 310016
- Publication Type:Journal Article
- Keywords:
Intestinal inflammation;
Deoxycholic acid;
Intestinal microbiota;
Bile acid;
Pyrosequencing;
High performance liquid chromatography-tandem mass spectrometr
- From:
Chinese Journal of Inflammatory Bowel Diseases
2021;05(1):77-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe and analyze the influence of deoxycholic acid (DCA) on intestinal microbiota and bile acid metabolism during the process of enteritis induced by DCA in mice.Methods:Twenty C57BL/6J mice were randomly and equally divided into DCA group and control group. The mice in DCA group were developed by feeding the feed with 0.2% DCA and the mice in control group were developed with routine feed for 24 weeks. The degree of intestinal tissue inflammation was evaluated by HE staining and scored, the change of intestinal microbiota was analyzed by pyrosequencing, bile acid levels of all grades in faeces were detected by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS) , the expressions of bile acid-related genes were detected by quantitive real-time PCR.Results:The intestinal histology scores in DCA group was significantly higher than that of the control group (both P<0.05) . The diversity of fecal microbiota in the DCA group was significantly reduced, the percentage of Firmicutes at the phylum level and the percentage of Clostridium XIVa at the genus level were significantly decreased (both P<0.05) . The total bile acid, secondary bile acid, unconjugated bile acid and tauro-α-muricholic acid (T-α-MCA) of mice faeces in DCA group were significant higher than those in control group (all P<0.05) . The expression of biliary acid transporter gene organic solute transporter β ( Ost- β) , farnesoid X receptor ( FXR) and fibroblast growth factor 15 ( FGF15) in DCA group decreased significantly (all P<0.05) , while the expressions of liver bile synthesis rate-limiting enzymes Cyp7a1, Cyp7b1 and Cyp27a1 increased obviously (all P<0.05) . Conclusions:DCA can induce the progress of enteritis, which may be related to its destruction of intestinal microbiota in mice and the promotion of liver bile acid synthesis through FXR-FGF15 signaling pathway.
