Chemical constituents from the root tubers of Stephania kwangsiensis and their tyrosinase inhibition and insecticidal activities
10.3969/j.issn.1001-1528.2024.10.021
- VernacularTitle:广西地不容块根化学成分及其酪氨酸酶抑制、杀虫活性研究
- Author:
Yan-ting NONG
1
;
Bi-qun ZOU
;
Si-wen ZENG
;
Ya-feng WANG
;
Bing-yuan YANG
;
Yong-lin HUANG
;
Lun-fa GUO
;
Ke-di YANG
;
Rui-jie HE
;
Li GE
Author Information
1. 广西大学医学院,广西 南宁530004;广西壮族自治区中国科学院广西植物研究所,广西植物功能物质与资源持续利用重点实验室,广西 桂林 541006
- Publication Type:Journal Article
- Keywords:
Stephania kwangsiensis H.S.Lo;
root tubers;
chemical constituents;
isolation and identification;
tyrosinase;
insecticidal activities
- From:
Chinese Traditional Patent Medicine
2024;46(10):3310-3315
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the root tubers of Stephania kwangsiensis H.S.Lo and their tyrosinase inhibition and insecticidal activities.METHODS The 70％ ethanol extract from root tubers of S.kwangsiensis was isolated and purified by Sephadex LH-20,MCI,ODS,semi-prepative HPLC and HSCCC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The tyrosinase inhibitory activities were determined by using levodopa as substrate,and the insecticidal activities were evaluated by the control effect of Diaphorina citri.RESULTS Twelve compounds were isolated and identified as tetrahydropalmatine ( 1 ),dehydrocrebanine ( 2 ),crebanine ( 3 ),stephanine ( 4 ),liriodenine ( 5 ),piperumbellactam A ( 6 ),sinoacutine ( 7 ),(+)-salutaridine N-oxide ( 8 ),bisnorargemonine ( 9 ),(+)-corytuberine (10),sebiferine (11) and palmatrubine (12).The IC50 values of compounds 5-7 to tyrosinase were (0.1702±0.0101),(0.7663±0.0331) and (0.5193±0.0075) mg/mL,respectively.The control effects of compounds 2-5,7,8,10-12 against D.citri ranged from ( 19.33±0.57 )％ to ( 77.15±0.45 )％.CONCLUSION Compounds 2,5,6,and 8-12 are isolated from this plant for the first time,6 and 9 are first obtained from genus Stephania.Compounds 5-7 displayed significant tyrosinase inhibition activities.Compounds 7,8 and 10 show strong insecticidal activities.
