Chemical constituents from the ethyl acetate fraction of branches of Morus nigra and their glucose uptake activities
10.3969/j.issn.1001-1528.2024.10.022
- VernacularTitle:黑桑枝乙酸乙酯部位化学成分及其葡萄糖摄取活性研究
- Author:
Yong-ke ZHOU
1
;
Yi-ting ZHANG
;
Wei GAO
;
Shi-wen KANG
;
Ke-jian PANG
;
Xin-zhou YANG
Author Information
1. 石河子大学药学院,新疆植物药资源利用教育部重点实验室,新疆 石河子832003
- Publication Type:Journal Article
- Keywords:
Morus nigra L.;
ethyl acetate fraction;
chemical constituent;
isolation and identification;
glucose uptake
- From:
Chinese Traditional Patent Medicine
2024;46(10):3316-3323
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the ethyl acetate fraction of the branches of Morus nigra L.and their glucose uptake activities.METHODS The extract was isolated and purified by silica gel,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.L6 cells were used to evaluate glucose uptake activities.RESULTS Sixteen compounds were isolated and identified as (R)-2-(3,4-dihydroxyphenyl)-3,5,9-trihydroxy-8,8-dimethyl-9,10-dihydropyranochromone ( 1 ),moracin C ( 2 ),nigranol C ( 3 ),nigragenon E (4),8-prenylquercetin (5),nigrasin I (6),sinopodophylline B (7),kuwanon C (8),gancaonin O (9),albanin A ( 10 ),6-O-methyl-moracin M ( 11 ),wittifuran W ( 12 ),8-hydroxy-2,2-dimethyl-2H,5H-pyrano[3,2-c]chromen-5-one (13),norartocarpanone (14),norartocarpetin (15),nigrasin D (16).The glucose uptake of compounds 4 and 13 was 1.97 times and 2.78 times that of the control group,respectively.CONCLUSION Compounds 1 is a novel natural product,5,7,9,12,13 and 15 are isolated from M.nigra for the first time.Compounds 4 and 13 can significantly promote the glucose uptake in L6 cells,showing strong hypoglycemic activities.
