Chemical constituents from the n-hexane fraction of Baccharis trimera from Brazil and their in vitro anti-liver injury activities
10.3969/j.issn.1001-1528.2024.12.022
- VernacularTitle:巴西产酒神菊正己烷部位化学成分及其体外抗肝损伤活性研究
- Author:
Xiong YANG
1
;
Hong-dong LIU
1
;
Tao JIANG
1
;
Jian-xin MIN
1
;
Bin LI
1
Author Information
1. 江西中医药大学院士工作站,江西 南昌330004
- Publication Type:Journal Article
- Keywords:
Baccharis trimera (Less.) DC;
chemical constituents;
GC-MS;
drug-containing serum;
LO2 cells;
antioxidant activities;
anti-liver injury
- From:
Chinese Traditional Patent Medicine
2024;46(12):4042-4048
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the n-hexane fraction of Baccharis trimera (Less.) DC from Brazil and their in vitro anti-liver injury activities.METHODS The chemical constituents and blood components were identified by GC-MS and LC-MS.The ethanol-induced LO2 cells were used to evaluate their anti-liver injury effects,and the cell survival rate and the levels of ROS,ALT,AST,GSH and GST were detected.RESULTS A total of54 compounds were isolated and identified from n-hexane fraction,including (-)-palustrol (8.55％),viridiflorol (6.83％),spathulenol (4.26％),and 7 prototype components such as caryophyllin and (-)-α-cubebene were identified from the drug-containing serum.Compared with the model group,the cell viability rate of drug-containing serum group was significantly increased (P<0.01),and the levels of ALT,AST and ROS were significantly decreased (P<0.01),and the levels of GST and GSH were significantly increased (P<0.05).CONCLUSION The n-hexane fraction from the Baccharis trimera has a certain in vitro antioxidant activity and hepatoprotective effects.It' s possible that sesquiterpenoids play a role in antioxidant activity.
