Simultaneous content determination of twelve constituents in Tufuling Qiwei Powder by HPLC-MS/MS
10.3969/j.issn.1001-1528.2024.12.002
- VernacularTitle:HPLC-MS/MS法同时测定土茯苓七味散中12种成分的含量
- Author:
Yu-xia HU
1
;
Jun LI
;
Yue-wu WANG
;
Xiao-li LÜ
;
Meng-di ZHANG
;
Nan LI
;
Tu-ya BAI
;
Fu-hou CHANG
Author Information
1. 内蒙古医科大学药学院,内蒙古 呼和浩特010110;内蒙古自治区新药筛选工程研究中心,内蒙古呼和浩特010110;内蒙古医科大学新药安全评价研究中心,内蒙古 呼和浩特010110
- Publication Type:Journal Article
- Keywords:
Tufuling Qiwei Powder;
chemical constituents;
content determination;
HPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(12):3906-3910
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC-MS/MS method for the simultaneous content determination of berberine,coptisine,gallic acid,corilagin,geniposide,toosendanin,rutinum,chlorogenic acid,luteolin,asiatica,emodin and astilbin in Tufuling Qiwei Powder.METHODS The analysis was performed on a 35℃ thermostatic Shim-pack GIST-HP C18 column (2.1 mm × 100 mm,3 μm),with the mobile phase comprising of methanol-water (containing 0.1％ formic acid) flowing at 0.25 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative and positive ion detection with multiple reaction monitoring mode.RESULTS Twelve constituents showed good linear relationships within their own ranges (r≥0.9990),whose average recoveries were 94.83％-104.42％ with the RSDs of 1.30％-4.88％.CONCLUSION This simple,rapid and accurate method can be used for the quality control of Tufuling Qiwei Powder.
