Exploration on the Effects of Wenjing Decoction on Autophagy in Endometriosis through PI3K/Akt/mTOR Pathway Based on Bioinformatics and Experimental Verification
10.19879/j.cnki.1005-5304.202406540
- VernacularTitle:基于生物信息学和实验验证探讨温经汤通过PI3K/Akt/mTOR通路对子宫内膜异位症自噬的影响
- Author:
Yi ZHANG
1
;
Lulu WU
;
Bing HE
;
Yingying LIANG
;
Li TANG
;
Quanning TAN
Author Information
1. 湖南中医药大学第二附属医院,湖南长沙 410005
- Publication Type:Journal Article
- Keywords:
Wenjing Decoction;
endometriosis;
bioinformatics;
PI3K/Akt/mTOR signaling pathway
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(1):60-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Wenjing Decoction in treating endometriosis(EMT)using bioinformatics methods and in vitro experiments.Methods The active components and corresponding targets of Wenjing Decoction were obtained from the TCMSP database,while EMT-related targets were identified using the GEO database.Functional enrichment analysis was conducted on the targets to predict core targets for treating EMT with Wenjing Decoction.Molecular docking was performed on core targets-drug ligands,and in vitro experiments validated the findings.Results Through screening the TCMSP database,117 active components of Wenjing Decoction were identified,corresponding to 248 targets;5 312 EMT-related differential genes were gathered from GEO database,identifying 97 potential targets of Wenjing Decoction for treating EMT,with core targets being IL6,TNF and EGFR.Functional enrichment analysis of EMT differential genes showed enrichment in pathways such as neuroactive ligand-receptor interaction,MAPK signaling pathway,endocytosis,calcium signaling pathway,autophagy and PI3K-Akt signaling pathway.Molecular docking showed that IL6,TNF,EGFR bind stably to their corresponding drug ligands.In vitro experiments indicated that Wenjing Decoction could inhibit the PI3K/Akt/mTOR pathway,promote LC3 Ⅰ to LC3 Ⅱ conversion,enhance the expression of Beclin-1,and reduce P62 expression.Moreover,Wenjing Decoction could hinder the expression of the endometriosis-specific biomarker CA125,decrease EGFR,IL-6 and TNF-α expressions in ectopic endothelial cells,inhibiting proliferation.Conclusion Wenjing Decoction can treat EMT through multiple pathways and targets,with the key mechanism being the reversal of autophagy inhibition via down-regulating of the PI3K/Akt/mTOR pathway.
