Preparation of curcumin-loaded PLGA nanoparticles and their effects on promoting wound healing
10.3969/j.issn.1001-1528.2024.12.006
- VernacularTitle:姜黄素PLGA纳米粒制备及其促进创面愈合作用
- Author:
Lan ZOU
1
;
Jin-jin XIE
1
;
Ru-jing WANG
1
;
Huan YANG
1
;
Xin DU
1
;
Dan-dan MI
1
;
San-jun SHI
1
Author Information
1. 成都中医药大学药学院,四川 成都611137
- Publication Type:Journal Article
- Keywords:
curcumin;
poly (D,L-lactide-co-glycolide) acid (PLGA) nanoparticles;
preparation process;
phacoemulsification method;
wound healing
- From:
Chinese Traditional Patent Medicine
2024;46(12):3930-3937
- CountryChina
- Language:Chinese
-
Abstract:
AIM To prepare curcumin-loaded poly (D,L-lactide-co-glycolide) acid (PLGA) nanoparticles,and to investigate their effects on promoting wound healing.METHODS Phacoemulsification method was adopted in the preparation of PLGA nanoparticles.With lactic acid-glycolic acid ratio,surfactant type and concentration and PLGA consumption as influencing factors,encapsulation efficiency,drug loading,particle size and PDI as evaluation indices,the preparation process was optimized by single factor test.The morphology was observed under transmission electron microscopy,after which the in vitro drug release was determined by dialysis bag method,the optical properties were investigated by fluorescence spectroscopy,and the effects on promoting wound healing of mouse skin.RESULTS The optimal conditions were determined to be 50∶50 for lactic acid-glycolic acid ratio,60 mg for PLGA consumption,and 3％ for surfactant (PVA) concentration.The obtained PLGA nanoparticles with spherical core-shell structure demonstrated the average particle size,PDI,Zeta potential and accumulative release rate within 168 h of (149.70±7.27) nm,0.28±0.04 and (-10.18±1.00) mV and (39.21±1.44)％,respectively,which displayed typical aggregation-induced emission property,excitation wavelength-dependent property and optical stability.Compared with the PBS group,PBS+illumination group,curcumin+illumination group and preparation group,the preparation+illumination group exhibited increased wound healing rate (P<0.05,P<0.01) and reduced wound epidermal thickness (P<0.05,P<0.01).CONCLUSION Curcumin-loaded PLGA nanoparticles show good optical properties,which can promote the wound healing of mouse skin when combined with photodynamic therapy.
