Detection and analysis of Liaoning virus from Aedes vexans collected in Hunchun city, Jilin Province
10.3760/cma.j.cn112866-20240522-00085
- VernacularTitle:吉林省珲春市刺扰伊蚊中辽宁病毒的检测与分析
- Author:
Yuhong YANG
1
;
Guangjun JIN
;
Bangshuai ZHANG
;
Weimin GOU
;
Qikai YIN
;
Kai NIE
;
Shihong FU
;
Qianqian CUI
;
Songtao XU
;
Fan LI
;
Xingzhou LI
;
Huanyu WANG
Author Information
1. 佳木斯大学公共卫生学院，佳木斯 154007
- Publication Type:Journal Article
- Keywords:
Mosquito-borne viruses;
Liaoning virus;
Aedes vexans
- From:
Chinese Journal of Experimental and Clinical Virology
2024;38(6):621-627
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study conducted mosquito-borne viruses RNA screening and analysis of virus evolution characteristics on mosquito specimens collected in 2023 from Hunchun city, Jilin province, China.Methods:Firstly, morphological method were employed for mosquito specimen classification. Then, real-time fluorescence quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the RNA of six mosquito-borne viruses in the collected mosquitos, i. e., Banna virus (BAV), Kadipiro virus (KDV), Liaoning virus (LNV), Tahyna virus (TAHV), Getah virus (GETV) and Japanese encephalitis virus (JEV). And by sequencing, the viral genome sequence of positive samples was obtained.Results:A total of 5490 mosquito specimens were collected from Hunchun city, Jilin province, included 4400 Aedes vexans (80.15%), 1090 Anopheles sinensis (19.85%). A total of 41 groups were obtained by mixing samples according to the time, location, and mosquito species collected. qRT-PCR result showed that only the Aedes vexans sample with the number JLHC2321 was tested positive for LNV, while the remaining samples were tested negative for the detected viruses. According to the phylogenetic analysis of the segment 10 gene, this LNV strain had the closest genetic relationship with NE9731 and belonged to the type II branch. Meanwhile, the amino acid sequence analysis based on the coding sequence (CDS) in the segment 10 showed that JLHC2321 only had 2 amino acid differential sites with the GII reference strain NE9731.Conclusions:This study detected LNV for the first time in Aedes vexans in Hunchun city, and our result provide important basic data for the monitoring and prevention strategies of mosquito-borne viruses in the region.
