Determination of 2-amino-5-chloro-N, 3-dimethylbenzamide in blood by ultra-performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry
10.3760/cma.j.cn121094-20240919-00432
- VernacularTitle:血液中2-氨基-5-氯-N，3-二甲基苯甲酰胺测定的UPLC-Q-Orbitrap HRMS法
- Author:
Peng WANG
1
;
Hua ZOU
;
Yuanqiang LU
;
Qiuliang XU
;
Lifang ZHOU
;
Xiangjing GAO
;
Hong REN
;
Yiyao CAO
Author Information
1. 浙江省疾病预防控制中心职业健康与辐射防护所，杭州 310051
- Publication Type:Journal Article
- Keywords:
Blood;
2-Amino-5-chloro-N, 3-dimethylbenzamide;
Ultra-performance liquid chromatography;
Orbitrap high resolution mass spectrometry;
Occupational exposure
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2025;43(11):872-875
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of 2-amino-5-chloro-N, 3-dimethylbenzamide in blood by ultra-performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry (UPLC-Q-Orbitrap HRMS) .Methods:In October 2022, 200 μl of blood samples were taken and 800 μl of acetonitrile was added. The samples were centrifuged at 3500 r/min (with a centrifugation radius of 6.6 cm) for 15 minutes. The supernatant was detected in the positive ion target tandem mass spectrometry (Target MS 2) mode and quantified by the external standard method. Methodological validation was also carried out for the established method. Results:The working curve of 2-amino-5-chloro-N, 3-dimethylbenzamide showed good linearity in the concentration range of 2.07-165.44 μg/L, with a correlation coefficient ( r) of 0.9994. The intra-day and inter-day relative standard deviations ( RSD) for the detection of 2-amino-5-chloro-N, 3-dimethylbenzamide were 2.6%-3.0% and 3.1%-3.5%, respectively. Analytical accuracy of 2-amino-5-chloro-N, 3-dimethylbenzamide quantification ranged from 101.0 % to 108.4 %, and the limit of detection and limit of quantification were 0.11 μg/L and 0.36 μg/L, respectively. The blood samples were able to be stored at -20 ℃ for at least 7 days. Conclusion:The UPLC-Q-Orbitrap HRMS determination method is sensitive, efficient and accurate, and can be used for the determination of 2-amino-5-chloro-N, 3-dimethylbenzamide in blood.
