Fast determination of maduramicin ammonium in serum by high performance liquid chromatography-tandem mass spectrometry
10.3760/cma.j.cn121094-20240518-00224
- VernacularTitle:血清中马杜霉素铵盐的高效液相色谱-串联质谱快速测定法
- Author:
Bo ZHOU
1
;
Bowen CHENG
;
Jing MA
;
Fang DONG
;
Ruichen LIU
;
Huiling LI
Author Information
1. 首都医科大学附属北京朝阳医院职业病与中毒医学科，北京 100020
- Publication Type:Journal Article
- Keywords:
Maduramicin ammonium;
Chromatography, liquid;
Tandem mass spectrometry;
High performance liquid chromatography-tandem mass spectrometry;
Serum
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2025;43(8):619-622
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the fast determination of maduramicin ammonium (MAD) in rat serum.Methods:In February 2024, rat serum samples were selected and directly injected after extraction and purification with methanol: acetonitrile (1: 1), separated on a C18 chromatographic column, and gradient-eluted using 0.1% formic acid aqueous solution -0.1% formic acid methanol solution as the mobile phase. Under optimized instrument conditions, electrospray positive ion multiple reaction monitoring (MRM) mode was employed for quantification using the external standard method, followed by methodological validation of the established approach.Results:The linearity of MAD in serum was good in the concentration range of 0.5-100 μg/L, and the correlation coefficient was 0.9997. The mean recoveries of MAD from spiked samples were 86.0%-109.6%, with the relative standard deviations were less than 10%. The limit of detection was 0.23 μg/L, The limit of detection was 0.75 μg/L.Conclusion:This method is high sensitive and reliable, which is suitable for the determination of MAD in in mouse serum.
