Determination of strychnine in human serum by liquid chromatography tandem mass spectrometry
10.3760/cma.j.cn121094-20240411-00160
- VernacularTitle:血清中毒鼠碱测定的液相色谱串联质谱法
- Author:
Xiaohong CHEN
1
;
Chen QIN
1
;
Jian ZHOU
1
Author Information
1. 宁波市疾病预防控制中心理化检验所，浙江省微量有毒化学物健康风险评估技术研究重点实验室，宁波 315010
- Publication Type:Journal Article
- Keywords:
Serum;
Chromatography, liquid;
Tandem mass spectrometry;
Strychnine
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2025;43(8):615-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of strychnine in human serum by liquid chromatography tandem mass spectrometry.Methods:In April 2024, the serum samples were extracted using alkaline ethyl acetate. Separation was achieved using a Waters HSS T3 column (100 mm×2.1 mm, 1.8 μm) with a linear gradient elution program of water/acetonitrile (containing 5 mmol/L ammonium formate and 0.1% formic acid) as the mobile phase. The analyte was detected by using electrospray ionization (ESI) in the positive multiple reaction monitoring (MRM) mode and quantified using an internal standard method.Results:A good linearity was obtained in the concentration range of 0.01~20.0 μg/L for target compound with the correlation coefficients≥0.999. The limitof detection (LOD) and the limit of quantitation (LOQ) were 0.003 μg/L and 0.01 μg/L, respectively. The average spiked recovery was 101.3%, the intra-day precision was 2.8%-5.4%, and the inter-day precision was 3.9%-6.3%, respectively.Conclusion:Liquid chromatogrphy tandem mass spectrometry method exhibits high sensitivity, strong specificity, and good accuracy, and can be used for the determination of strychnine in Homo sapiens serum.
