The method of determination for trichloroaniline in the air of workplace by liquid chromatography with solvent elution
10.3760/cma.j.cn121094-20240313-00095
- VernacularTitle:工作场所空气中三氯苯胺测定的溶剂洗脱-液相色谱法
- Author:
Bingben CHEN
1
;
Sanyan ZHENG
1
;
Shuran YANG
1
;
Xiaoli FANG
1
;
Ruifen LI
1
Author Information
1. 温州市疾病预防控制中心（温州市卫生监督所）理化检验所，温州 325000
- Publication Type:Journal Article
- Keywords:
Chromatography, liquid;
Trichloroaniline;
Air;
Workplace
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2025;43(7):539-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a solvent elution-liquid chromatography method for the determination of trichloroaniline concentration in the air of the workplace.Methods:From December 2023 to January 2024, 2, 4, 5-trichloroaniline, 2, 4, 6-trichloroaniline, and 3, 4, 5-trichloroaniline in the air were collected using glass fiber filter membranes. After elution with acetonitrile and filtration with a 0.22 μm mixed cellulose filter membrane, isometric elution with acetonitrile and water (volume ratio 55∶45) was performed using a C8 column (250 mm×4.6 mm×5 μm) , and detection was carried out using a photo-diode array (PDA) detector. The detection limit, precision and other indicators of the method were analyzed.Results:The separation and determination of trichloroaniline isomers were completed within 9 minutes by the solvent elution-liquid chromatography method. The linear range was 0.1-30 μg/ml, the detection limit of the method was 0.005 μg/ml, the recoveries were 88%-98%, and the relative standard deviation was 0.3%-3.2%.Conclusion:The solvent elution-liquid chromatography method for the determination of trichloroaniline is simple and efficient. Both the recovery and precision meet the standard requirements for the determination of toxic substances in the workplace, and it is suitable for the detection of trichloroaniline in the workplace.
