Non-invasive prenatal screening in three cases of vanishing twin syndrome and a literature review
10.3760/cma.j.cn511374-20250603-00336
- VernacularTitle:双胎之一消亡无创产前检测3例并文献复习
- Author:
Xinni SHU
1
;
Jiexia YANG
;
Yousheng WANG
;
Zhuangping ZHANG
;
Fangfang GUO
;
Haishan PENG
;
Dongmei WANG
;
Yaping HOU
Author Information
1. 广东省妇幼保健院医学遗传中心，广州 511442
- Publication Type:Journal Article
- Keywords:
Vanishing twin syndrome;
Noninvasive prenatal testing;
Cell-free fetal DNA;
Twin pregnancy;
Chorionicity
- From:
Chinese Journal of Medical Genetics
2025;42(7):855-861
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To assess the impact of vanishing twin syndrome (VTS) on the accuracy of non-invasive prenatal testing (NIPT).Methods:Three pregnant women who underwent NIPT testing at Guangdong Women and Children′s from November 2019 to February 2020 were selected as the study subjects. The three women had either vanish twin syndrome or had undergone fetal reduction for other reasons in one of their twins, and were subsequently subject to NIPT, chromosome karyotyping, chromosome microarray analysis (CMA), and short tandem repeat (STR) analysis. This study has been approved by the Medical Ethics Committee of Guangdong Maternal and Child Health Hospital (Ethic No.: 20230132).Results:Case 1 underwent selective fetal reduction at 8 + weeks of gestation. At 17 + weeks, NIPT showed a fetal DNA fraction of 2.806%, with results indicating the presence of Y chromosome and abnormal sex chromosome ratios. However, the women had subsequent uncomplicated vaginal delivery of a female infant, and no abnormality noted. Case 2 experienced spontaneous demise of one twin at 13 weeks′ gestation. At 19 weeks, NIPT indicated a high risk for chromosome 21 ( Z-score 4.671) in the surviving fetus, but subsequent evaluation showed no abnormality. Case 3, a dichorionic diamniotic (DCDA) twin pregnancy, underwent selective reduction at 13 + weeks due to fetal abnormalities in one twin. At 22 + weeks, NIPT for the surviving fetus indicated a high risk for chromosome 21 (Z-score 17.549), but subsequent evaluation was unremarkable. Conclusion:In twin pregnancies, the relatively low cell-free fetal DNA (cffDNA) concentration can compromise the success rate and accuracy of NIPT compared to singleton pregnancies. Residual DNA from the demised fetus may persist for weeks following VTS or selective reduction, potentially causing false-positive NIPT results and interfering with sex chromosome prediction for the surviving fetus. Additionally, determining chorionicity is critical for reliable interpretation of NIPT results in twin pregnancies.
