Application of a modified resazurin method for rapid detection of eravacycline susceptibility in Acinetobacter baumannii
10.3760/cma.j.cn112309-20240609-00204
- VernacularTitle:改良刃天青法在鲍曼不动杆菌依拉环素敏感性快速检测中的应用
- Author:
Fengli YUAN
1
;
Zitong PAN
1
;
Henan LI
1
Author Information
1. 北京大学人民医院检验科，北京 100044
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
Eravacycline;
Resazurin;
Rapid detection
- From:
Chinese Journal of Microbiology and Immunology
2025;45(6):493-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the resazurin reduction method for rapid clinical detection of eravacycline susceptibility of Acinetobacter baumannii. Methods:The susceptibility of 103 Acinetobacter baumannii strains to eravacycline was tested using the modified resazurin method and compared with the results from the broth microdilution method to evaluate the effectiveness of the improved method. Results:All results were obtained after about 5.5 h of incubation. The testing procedure was simple and saved 10-14 h compared with the broth microdilution method. The consistency in susceptibility result with the broth microdilution method was 99.03%. The very major error rate was 0.97%(1/103), with one resistant strain misclassified as sensitive (minimum inhibitory concentration of 2 μg/ml), and the major error rate was 0. This method could rapidly and accurately screen for eravacycline-resistant Acinetobacter baumannii strains in clinical settings. Conclusions:The modified resazurin method, due to its simplicity, rapidity, and ease of execution, has the potential to become a convenient method for the rapid clinical screening of eravacycline-resistant strains, providing a reference for early clinical treatment.
