Protective effect of myrislignan on autoimmune hepatitis in mice
10.3760/cma.j.cn112309-20240815-00304
- VernacularTitle:肉豆蔻木脂素对小鼠自身免疫性肝炎的保护作用
- Author:
Xueyang SUN
1
;
Wenbo LI
1
;
Lin WANG
1
;
Zhihong LIU
1
;
Junfeng ZHANG
1
;
Fenglian YAN
1
;
Hui ZHANG
1
Author Information
1. 济宁医学院基础医学院免疫学与分子医学研究所，济宁 272067
- Publication Type:Journal Article
- Keywords:
Autoimmune hepatitis;
Myrislignan;
Concanavalin A;
Macrophage
- From:
Chinese Journal of Microbiology and Immunology
2025;45(11):920-927
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of myrislignan（MRL）on concanavalin A（Con A）-induced autoimmune hepatitis（AIH）.Methods:C57BL/6J mice were divided into the following groups using a random number table，with five mice in each group：control group，MRL group，model group（Con A group），and MRL pretreatment group（MRL+Con A group）. MRL was injected intraperitoneally at a dose of 30 μg/g；3 h after pretreatment，Con A（18 μg/g）was administrated by intravenous injection；mouse livers and serum samples were collected 12 h after injection for measuring serum transaminase levels and liver cell apoptosis. The mRNA and protein expression levels of IL-6，IL-12，and TNF-α were measured using qRT-PCR and ELISA. Flow cytometry was performed to detect the proportion and activation status of macrophages in liver tissues. Bone marrow-derived macrophages（BMDMs）were isolated and induced in vitro to analyze the regulatory effect of MRL on macrophages. One-way analysis of variance was used to compare the differences in various indicators among groups. Results:Compared with the Con A group，MRL（30 μg/g）pretreatment significantly reduced alanine aminotransferase（ P<0.05）and aspartate transaminase（ P<0.01）levels，attenuated liver oxidative stress（increased superoxide dismutase activity，while decreased levels of malondialdehyde and myeloperoxidase；all P<0.05），and suppressed hepatocyte apoptosis（ P<0.01）. Both in vivo and in vitro experiments confirmed that MRL（30 μg/g）could reduce the proportion of M1 macrophages（ in vivo： P<0.05； in vitro：all P<0.001）and inhibit macrophage activation（ in vivo： P<0.01； in vitro：all P<0.05）. Conclusion:MRL effectively prevents Con A-induced autoimmune hepatitis by inhibiting liver cell apoptosis，attenuating liver oxidative stress，suppressing M1 macrophage polarization，and reducing inflammatory cytokine expression.
