Identification of the secretion of effector proteins of Chlamydia psittaci using the β-lactamase translocation assay
10.3760/cma.j.cn112309-20250110-00009
- VernacularTitle:基于β-内酰胺酶转位试验鉴定鹦鹉热衣原体效应蛋白的分泌
- Author:
Huiying YANG
1
;
Nana LI
;
Shan ZHANG
;
Yufei JANG
;
Yinhui LIN
;
Xiaoxiao CHEN
;
Yuchen ZHANG
;
Yonghui YU
;
Xuan OUYANG
;
Yajun SONG
;
Jun JIAO
Author Information
1. 牡丹江医科大学公共卫生学院，牡丹江 157011
- Publication Type:Journal Article
- Keywords:
Chlamydia psittaci;
Effector protein;
β-lactamase translocation assay;
Intracellular localization
- From:
Chinese Journal of Microbiology and Immunology
2025;45(9):761-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify and validate secreted effector proteins of Chlamydia psittaci ( C. psittaci) through bioinformatic prediction and experimental verification, and to characterize their subcellular localization in host cells. Methods:Potential effector proteins were predicted using bioinformatics tools. Candidate effectors were fused to β-lactamase through the constructed expression vectors, and these vectors were transformed into C. psittaci. The secretion of these candidate effectors was evaluated by β-lactamase translocation assays. Eukaryotic expression vectors of confirmed effectors were transfected into host cells to determine their intracellular localization patterns. Results:Bioinformatic analysis identified 29 candidate effector proteins. Experimental validation confirmed the secretion of five effectors, with four exhibiting cytoplasmic localization and one displaying nuclear localization in host cells.Conclusion:This study characterizes five novel C. psittaci secreted effector proteins, providing critical insights for investigating the molecular pathogenesis of psittacosis.
