Comparative study on the delivery efficacy of Yersinia pestis protective antigens mediated by different carrier proteins in the outer membrane vesicles of Escherichia coli
10.3760/cma.j.cn112309-20241121-00399
- VernacularTitle:不同载体蛋白在大肠埃希菌外膜囊泡上递送鼠疫耶尔森菌保护性抗原的比较研究
- Author:
Xiangting ZHANG
1
;
Xiangze MENG
;
Yuanning WANG
;
Yajing LYU
;
Yu ZHANG
;
Yiqian WANG
;
Zongmin DU
Author Information
1. 牡丹江医科大学公共卫生学院，牡丹江 157011
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
Outer membrane vesicles;
Antigen delivery;
Plague vaccine
- From:
Chinese Journal of Microbiology and Immunology
2025;45(8):687-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the efficiency and characteristics of different carrier proteins and signal sequences in delivering antigens into Escherichia coli outer membrane vesicles (OMVs). Methods:The fusion protein F1V, which consisted of the main protective antigen of Yersinia pestis F1 and LcrV, was expressed using the carrier proteins such as cytolysin A (ClyA), outer membrane protein A (OmpA), or β-lactamases (Bla) signal sequence as a carrier protein. The expression, localization, and content of F1V protein in OMVs were compared and analyzed. Results:All three delivery methods successfully incorporated F1V protein into OMVs and localized it on the surface of OMVs. Notably, when OmpA was used as the carrier protein, the F1V fusion protein constituted up to 30% of the total protein in OMVs. The highest yield of OMVs, reaching 4.2 mg/L, was achieved when Bla signal sequence was used as the carrier.Conclusions:There is a significant difference in the efficiency of different carrier proteins in delivering the F1V antigen into OMVs of Escherichia coli. Considering both the yield of OMVs and the proportion of antigen in the total protein of OMVs, the carrier Bla signal sequence demonstrated the highest efficiency in delivering F1V into OMVs, showing a potential for the future development of OMVs-based plague vaccines.
