Role of autophagy in regulating phagocytosis in Vibrio vulnificus-infected murine macrophages
10.3760/cma.j.cn112309-20240819-00308
- VernacularTitle:自噬对小鼠巨噬细胞吞噬创伤弧菌能力的影响
- Author:
Na CHEN
1
;
Yelin JIANG
;
Danli XIE
;
Xianhui HUANG
;
Yongliang LOU
;
Chaowei WEN
Author Information
1. 杭州市临平区第一人民医院检验科，杭州 311199
- Publication Type:Journal Article
- Keywords:
Autophagy;
Macrophage;
Phagocytosis;
Vibrio vulnificus
- From:
Chinese Journal of Microbiology and Immunology
2025;45(2):108-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of autophagy in the regulatory of phagocytosis in Vibrio vulnificus ( V. vulnificus)-infected murine macrophages. Methods:The expression of cellular autophagy-related proteins in PBS-treated and V. vulnificus-infected RAW264.7 and BMMφ cells was detected by Western blot. The co-localization of V. vulnificus-GFP and LC3Ⅱ protein in V. vulnificus-GFP-infected RAW264.7 and BMMφ cells were detected using confocal microscopy. The phagocytosis of V. vulnificus in V. vulnificus-GFP-infected RAW264.7 and BMMφ cells with or without autophagy inhibition using Bafilomycin A1 was detected by flow cytometry. Results:The up-regulated levels of Atg7, Atg12 and Atg16L1 proteins, increased LC3Ⅱ/actin ratio, as well as down-regulated p62 protein levels were significantly detected in V. vulnificus-infected RAW264.7 and BMMφ cells. The co-localization of V. vulnificus-GFP and LC3Ⅱ protein was clearly observed in V. vulnificus-GFP-infected RAW264.7 and BMMφ cells. Enhanced phagocytosis of V. vulnificus and increased autophagy were exhibited in V. vulnificus-GFP-infected RAW264.7 and BMMφ cells, while weakened phagocytosis, accumulation of Atg7, Atg12, Atg16L1, LC3Ⅱ and p62 protein levels, as well as blocking autophagy flux were detected in those cells within autophagy inhibition using Bafilomycin A1. Conclusion:Autophagy induced by V. vulnificus infection could promote phagocytosis of V. vulnificus in macrophages.
