Analysis of gut microbiota characteristics in elderly patients with sarcopenic obesity based on 16S rRNA sequencing
10.3760/cma.j.issn.0254-9026.2025.08.016
- VernacularTitle:基于16S rRNA测序对老年肌少性肥胖患者的肠道菌群特征分析
- Author:
Ling WANG
1
;
Xiangfeng HE
;
Yanqing REN
;
Yanping SONG
;
Lin MA
;
Nan CHEN
Author Information
1. 上海健康医学院附属崇明医院康复医学科，上海 202150
- Publication Type:Journal Article
- Keywords:
Sarcopenia;
Obesity;
16S;
Sarcopenic Obesity;
Gut Microbiota;
16S rRNA Sequencing
- From:
Chinese Journal of Geriatrics
2025;44(8):1114-1121
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the characteristics of gut microbiota changes in individuals with Sarcopenic Obesity(SO)based on 16S rRNA sequencing.Methods:This cross-sectional study was conducted in Chongming District, Shanghai from April to November 2021.Fecal samples were collected from 20 elderly SO patients (case group)and 40 elderly non-SO individuals(control group)for 16S rRNA sequencing in order to analyze the diversity, structural composition, and species differences of gut microbiota, and then to predict the differential metabolic functions of the gut microbiota.Results:A total of 60 subjects were included.The case group consisted of 20 individuals(15 males and 5 females)with an average age of 73.15 ± 4.09 years; the control group included 40 individuals(20 males and 20 females)with an average age of 71.20 ± 4.12 years.The α-diversity analysis revealed that the richness indices ACE and Chao 1 of the case group were significantly lower than those of the control group( P<0.05), while the diversity indices Shannon and Simpson showed a trend of being lower in the case group, but the differences were not statistically significant( P>0.05). Principal coordinate analysis based on the Unweighted-unifrac distance metric demonstrated a statistically significant difference in β-diversity between the two groups( P=0.003). The structure and composition of the gut microbiota in the case group were altered, with a significant reduction in the relative abundance of the Blautia genus in the case group( P<0.05). LEfSe analysis identified 5 and 16 enriched microbial species in the case and control groups, respectively (Linear Discriminant Analysis score >2, P<0.05). Additionally, PICRUSt2 functional prediction revealed significant differences( P<0.05)in metabolic pathways between the two groups, including quorum sensing, fat digestion and absorption, and folate biosynthesis. Conclusions:The gut microbiota in elderly SO patients is disordered, mainly manifested as a decrease in diversity and characteristic changes in structural composition, as well as a reduction in the abundance of the beneficial bacterium Blautia.The Progression of SO is closely associated with gut microbiota metabolic disturbances, and targeting the gut microbiota is expected to become a novel therapeutic approach for SO.
