Whole-genome sequence analysis of coxsackievirus A10 isolates in Anhui Province in 2018
10.3760/cma.j.cn112309-20240419-00138
- VernacularTitle:2018年安徽省柯萨奇病毒A组10型分离株全基因组序列分析
- Author:
Yonglin SHI
1
;
Yinuo LIU
;
Yinglu GE
;
Wanwan MA
;
Yong SUN
;
Jiabing WU
Author Information
1. 安徽省疾病预防控制中心（安徽省公共卫生研究院），安徽省医疗卫生重点专科病原微生物分子生物学实验室，合肥 230601
- Publication Type:Journal Article
- Keywords:
Coxsackievirus;
Whole-genome sequence;
Phylogenetic analysis
- From:
Chinese Journal of Microbiology and Immunology
2025;45(4):341-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To characterize the molecular evolution of coxsackievirus A10 (CVA10) strains in Anhui Province.Methods:The nucleic acids of CVA10 isolates in Anhui Province were extracted for whole-genome PCR amplification. One-generation sequencing was performed and the complete whole-genome sequences of 10 isolates were obtained. Nucleotide and amino acid sequence similarity analysis of CVA10 isolates and reference strains was performed using MegAlign in DNAStar software. MEGA 11.0 was used to classify the genotypes of CVA10 isolates and representative strains based on phylogenetic analysis of the VP1 region, and the average evolutionary differences between genotypes were calculated. BioEdit 7.2 was used to calculate the entropy of amino acid substitution in the P1-P3 region of the isolates and analyze the amino acid non-synonymous substitution sites. Recombination signals associated with the Anhui isolates were detected using RDP4 and further verified using Simplot 3.5. DnaSp6 software was selected to analyze the selection pressure of CVA10 isolates and prototype strains.Results:Based on the VP1 region, CVA10 isolates and CVA10 representative strains were categorized into A-F genotypes, and most of the CVA10 prevalent in mainland China belonged to the F genotype, with some isolates in Anhui Province being more closely related to the Yunnan isolates. The average rate of evolutionary difference in nucleotides among genotypes ranged from 18.70% to 33.70%. The isolates shared 17 non-synonymous amino acid mutation sites in the VP1 region, and amino acid substitutions in the VP1, 3A and 3C regions might affect the pathogenicity of the strains. The isolates frequently recombined with a variety of other EVA strains in the 5′UTR, 2C, 3C, 3D, and 3′UTR regions. Selection pressure analysis of the isolates showed that the isolate genes were affected by negative selection pressure.Conclusions:Genetic evolutionary analysis of CVA10 suggests that mutations and recombination with other types of EVA strains are prevalent, affecting the molecular epidemiological trend of CVA10, and that molecular surveillance of CVA10 strains in Anhui Province should continue to be strengthened.
