Recognition by the POTRA domain is an essential determinant to initiate the biogenesis of outer membrane proteins for Omp85 family proteins
10.3760/cma.j.cn112309-20250311-00079
- VernacularTitle:Omp85家族蛋白中POTRA结构域是外膜蛋白组装起始的关键
- Author:
Xiaochen HAN
1
;
Qingrong LI
1
;
Qing WANG
1
;
Leyi ZHAO
1
;
Hanqing ZHANG
1
;
Youcai QIN
1
;
Enguo FAN
1
;
Yindi CHU
1
Author Information
1. 中国医学科学院基础医学研究所北京协和医学院基础学院病原学系，北京 100005
- Publication Type:Journal Article
- Keywords:
Outer membrane Protein;
Omp85 superfamily;
FhaB/FhaC;
TtOmp85;
In vitro reconstitution system
- From:
Chinese Journal of Microbiology and Immunology
2025;45(5):373-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the essential determinants that are critical to initiating the assembly of outer membrane proteins by replacing the POTRA domains of the translocator protein FhaC and the insertase protein TtOmp85 of the Omp85 family. Methods:FhaC, TtOmp85 proteins and their recombinant chimeric proteins after replacing the POTRA domain were obtained by overexpression and purification in vitro. An in vitro reconstitution system was used to investigate the effects of the different domains on the transport efficiency of the substrate outer membrane protein FhaB and the membrane insertion efficiency of OmpA. Results:Replacing the POTRA domain of FhaC with that of TtOmp85 led to the loss of the transport function of FhaB. During the membrane insertion process of OmpA, the FhaC mutant containing the POTRA of TtOmp85 protein acquired the ability to assemble OmpA. Conclusion:The compositional differences in the POTRA domain of Omp85 family proteins determine their abilities to recognize their substrate proteins.
