Dynamics of HIV reservoir and α4β7 cells in patients with different immune reconstitution outcomes during long-term antiretroviral therapy
10.3760/cma.j.cn112309-20241222-00438
- VernacularTitle:长期抗逆转录病毒治疗过程中不同免疫重建结局的HIV储存库和α4β7细胞动态变化研究
- Author:
Lingyu GAO
1
;
Xueji LI
;
Yanmei JIAO
;
Mengmeng QU
;
Zheng XU
;
Jijing SHI
;
Baopeng YANG
;
Luxue ZHANG
Author Information
1. 郑州市第一人民医院干细胞转化与应用医学重点实验室，郑州 450000
- Publication Type:Journal Article
- Keywords:
Human immunodeficiency virus;
Antiretroviral therapy;
Virus reservoir;
α4β7 cell;
Immunological non-responder
- From:
Chinese Journal of Microbiology and Immunology
2025;45(4):285-292
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate immunological non-responders (INRs) and immunological responders (IRs) during long-term antiretroviral therapy (ART), and study the dynamics of HIV reservoir and α4β7 cells in INRs and IRs and their correlation.Methods:Twenty-six patients with chronic HIV infection who received ART for 5 years were included. They were divided into INRs (CD4 + T cell counts≤350 cells/μl, n=9) and IRs (CD4 + T cell counts≥500 cells/μl, n=17) based on immune reconstitution outcomes. The percentages and numbers of α4β7 cells in both groups at baseline, ART 1, 3, and 5 years were detected by flow cytometry, and the levels of HIV DNA and cell-associated HIV RNA were quantified by real-time fluorescent quantitative PCR during the same periods. HIV viral decay, α4β7 cells dynamics, and their correlations with T cells were compared at baseline, ART 1, 3 and 5 years between the two groups. Results:Over 5 years of ART, INRs exhibited higher HIV reservoir levels compared to IRs, but the decline trend was not slow. The counts of α4β7 cell were lower and the growth trend was slow in INRs ( P<0.05). α4β7 cell counts were strongly positively correlated with CD4 + T cell counts at all timepoints (Year 1: r=0.887; Year 3: r=0.878; Year 5: r=0.887; P all <0.001), while showing significantly negative correlations with activated CD38 + HLA-DR + CD4 + T cells (Year 1: r=-0.619, P=0.001), CD38 + HLA-DR + CD8 + T cells (Year 1: r=-0.517; Year 5: r=-0.532; P all <0.01), and PD-1 + CD4 + T cells (Year 1: r=-0.476, Year 5: r=-0.390, P all <0.05). Conclusions:During long-term ART, INRs maintained higher HIV reservoir and lower α4β7 cell counts compared with IRs, and decreased α4β7 cells may be associated with disease progression.
