Molecular epidemiological characteristics of group B Streptococcus from puerpera based on whole genome sequencing
10.11816/cn.ni.2025-258119
- VernacularTitle:基于全基因组测序B族链球菌孕产妇分离株的分子流行病学特征
- Author:
Zhibo TAO
1
;
Anjun CHEN
;
Yiqing TAO
;
Yongqin GUO
;
Yangyang HAO
;
Peng LIU
;
Yang LIU
;
Dandan WEI
Author Information
1. 南昌大学第一附属医院检验中心,江西南昌 330052
- Publication Type:Journal Article
- Keywords:
Group B Streptococcus;
Whole genome sequencing;
Serotype;
Drug resistance gene;
Virulence gene;
Epidemiology;
Isolates from puerpera
- From:
Chinese Journal of Nosocomiology
2025;35(22):3410-3414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To understand the drug resistance,serotypes,virulence-associated genes and epidemiologi-cal characteristics of group B Streptococcus(GBS)isolated from puerpera in this area so as to provide bases for prevention of mother-to-infant infections.METHODS Totally 67 strains of GBS were isolated from obstetrics out-patient department of The First Affiliated Hospital of Nanchang University from Jan.2023 to Dec.2023.The spe-cies of the strains were identified by VITEK MS,the drug susceptibility testing was carried out by disc diffu-sion method.Multilocus sequencing types,capsular types,virulence genes and drug resistance genes were analyzed by means of whole genome sequencing technique.RESULTS The 67 strains of GBS were sensitive to penicillin,vancomycin,ceftriaxone and linezolid;the drug resistance rates to erythromycin and clindamycin were 76.12％and 55.22％,respectively.All strains fell into 7 serotypes,with serotype V predominant;21 sequence types were in-volved,with ST529 most prevalent;8 clonal complexes(CCs)were involved,with CC12 most common.Totally 17 types of drug resistance genes were identified,and the carrying rate of macrolide resistance gene ErmB was highest.Among all the virulence genes except for the adhesion genes fbsA and fbsB,the carrying rates of 18 genes involving in invasion,adhesion,and immune evasion-associated virulence genes were more than 86.57％;67.16％of the strains co-expressed both PI-1 and PI-2a pilus islands.CONCLUSIONS The drug resistance rate of the GBS strains isolated from the puerpera is high,and the strains carry multiple drug resistance genes and viru-lence genes and present with molecular clonal diversity.The serotype V/ST529 is the predominant clone,for which the prevention and control should be strengthened.
