Effects of Yixintai on Lipid Metabolism and Protein Expressions of CPT-1 and CD36 in Rats with Heart Failure
10.19879/j.cnki.1005-5304.202504582
- VernacularTitle:益心泰对心力衰竭大鼠脂代谢及CPT-1、CD36蛋白表达的影响
- Author:
Yingli ZHOU
1
;
Xi LUO
;
Ziwei YIN
;
Huizhen ZHENG
;
Jiaming WEI
;
Ziyan WANG
;
Chengxin LIU
;
Ya LI
;
Zhihua GUO
Author Information
1. 湖南中医药大学第一中医临床学院,湖南 长沙 410208
- Publication Type:Journal Article
- Keywords:
Yixintai;
heart failure;
lipid metabolism;
CPT-1;
CD36;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(12):82-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Yixintai on lipid metabolism and the protein expressions of CPT-1 and CD36 in rats with heart failure;To explore the mechanism of its treatment of heart failure.Methods 106 out of 120 SD rats were selected to establish the heart failure model induced by myocardial infarction by ligating the left anterior descending coronary artery,and 14 rats were selected as the sham-operation group.The successful model rats were randomly divided into model group,trimetazidine group and Yixintai low-,medium-and high-dosage groups,with 14 rats in each group.The administration group was given corresponding drugs by gavage once a day for 4 weeks.LVEF,LVFS,LVIDd and LVIDs were measured by color doppler ultrasonography,the contents of ANP,BNP,LA and FFA in serum were detected by ELISA,the contents of TG,TC,LDL and HDL were detected by automatic biochemical analyzer,HE and Masson staining were used to observe the morphology of myocardial tissue,the expressions of CPT-1 and CD36 protein in myocardial tissue were detected by Western blot.Results Compared with the sham-operation group,LVEF and LVFS in the model group decreased(P<0.05),the LVIDs and LVIDd increased(P<0.05);the contents of serum ANP,BNP,LA,FFA,TG,TC and LDL increased(P<0.05),while the content of HDL decreased(P<0.05),with myocardial edema,irregular arrangement of myocardial fibers,increased inflammatory cell infiltration and collagen fiber deposition;the protein expressions of CPT-1 and CD36 in myocardial tissue decreased(P<0.05).Compared with the model group,the LVEF and LVFS in Yixintai each dosage groups and trimetazidine group increased(P<0.05),LVIDs and LVIDd decreased(P<0.05);the contents of ANP,BNP,LA,FFA,TG,TC and LDL in serum of Yixintai medium-and high-dosage groups and trimetazidine group decreased(P<0.05),the content of HDL increased(P<0.05);myocardial edema was improved,inflammatory cell infiltration was reduced,collagen fiber deposition was reduced,and the protein expressions of CPT-1 and CD36 in myocardial tissue increased(P<0.05).Conclusion Yixintai may improve myocardial energy metabolism and treat heart failure by increasing the expression of CPT-1 and CD36 protein in myocardial tissue and promoting fatty acid β oxidation.
