Exploration on Mechanism of Tanyu Tongzhi Optimized Prescription in Anti-atherosclerosis Based on Network Pharmacology and Experimental Validation
10.19879/j.cnki.1005-5304.202505257
- VernacularTitle:基于网络药理学及实验验证探究痰瘀同治优化方治疗动脉粥样硬化作用机制
- Author:
Lingfeng ZHOU
1
;
Yingying LI
;
Danli TANG
;
Huamin ZHANG
;
Xiang LI
Author Information
1. 中国中医科学院医学实验中心,北京 100700
- Publication Type:Journal Article
- Keywords:
Tanyu Tongzhi Optimized Prescription;
atherosclerosis;
network pharmacology;
molecular docking;
experimental verification
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(12):30-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the pharmacodynamic material basis and mechanism of Tanyu Tongzhi Optimized Prescription in the treatment of atherosclerosis(AS)using network pharmacology and animal experiments.Methods UPLC-Q-TRAP-MS/MS was used to identify the blood-entering components of Tanyu Tongzhi Optimized Prescription and conduct network pharmacological analysis.Important components and their core targets for the treatment of AS were screened,and molecular docking was conducted.The AS model mice were constructed and treated with Tanyu Tongzhi Optimized Prescription.HE staining and oil red O staining were used to observe aortic tissue morphology.The blood lipid status was detected by an automatic blood biochemical analyzer.The contents of serum inflammatory factors were detected by ELISA,and the mRNA expressions of aortic core targets were detected by RT-qPCR.Results It was identified and predicted that 30 blood-entering components of Tanyu Tongzhi Optimized Prescription might exert therapeutic effects on AS by regulating core targets such as IL6,TNF,IL1B,AKT1 and NFKB1,and regulating the TNF signaling pathway,Toll-like receptor signaling pathway,PI3K-Akt signaling pathway,etc.Animal experiments showed that Tanyu Tongzhi Optimized Prescription could reduce aortic plaque area and inflammatory cell infiltration,alleviate lipid deposition and vascular stenosis,improve blood lipid levels,and significantly reduce the serum contents of TNF-α,IL-1β,and mRNA expressions of TNF-α,IL-6,AKT1 and NF-κB in aortic tissue(P<0.05,P<0.01).Conclusion The effect of Tanyu Tongzhi Optimized Prescription may regulate TNF,Toll-like receptor and other signaling pathways by acting on core targets such as IL6,TNF,IL1B,and inhibit inflammatory response,so as to treat AS.
