Molecular epidemiological characteristics of clinical Haemophilus influenzae isolates from adults and children with community-acquired pneumonia
10.11816/cn.ni.2025-250595
- VernacularTitle:成人与儿童社区获得性肺炎临床分离流感嗜血杆菌分子流行特征
- Author:
Qian SHI
1
;
Hefei ZHA
;
Haiye WANG
;
Xueli ZHANG
;
Xin ZHANG
Author Information
1. 新疆生产建设兵团医院/石河子大学医学院第二附属医院检验科,新疆乌鲁木齐 830002;兵团医学检验临床医学研究中心,新疆乌鲁木齐 830002
- Publication Type:Journal Article
- Keywords:
Haemophilus influenzae;
Community-acquired pneumonia;
Multilocus sequencing typing;
Drug resist-ance;
Adult;
Child
- From:
Chinese Journal of Nosocomiology
2025;35(21):3276-3281
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the molecular epidemiological characteristics of clinical Haemophilus influ-enzae(Hin)isolates from adults and children with community-acquired pneumonia(CAP)so as to provide theo-retical bases for effective prevention and treatment of Hin infection.METHODS The patients who were hospital-ized in respiratory and critical care medicine department and pediatrics department of Xinjiang Production and Con-struction Corps Hospital due to CAP from Jan.2023 to Dec.2024 were enrolled in the study,and the positive rates of Hin in sputum specimens were statistically analyzed.The clinical distribution and results of drug suscepti-bility testing for Hin strains were observed and compared between the adults with CAP and the children with CAP.The capsular types,drug resistance genes and multilocus sequencing typing(MLST)subtypes were detected for 72 strains of Hin.RESULTS The positive rate of Hin was higher among the children with CAP(9.46％)than among the adults with CAP(2.71％).The Hin was more prevalent among the children with CAP than among the adults,it was highly prevalent in autumn and winter,with the population dominated by the children aged between 13 and 17 years old(12.67％).The positive rate of β-lactamase-producing Hin strains was 74.71％among the a-dult patients and 90.17％among the children,respectively;the drug resistance rates to ampicillin were highest(73.86％and 92.57％),the drug resistance rates of the strains isolated from the children to the two types of β-lac-tams were higher than those of the strains isolated from the adults(P＜0.05).The non-typeable Hin strains(94.45％)were the predominant type among the 72 strains of Hin,the production of β-lactamase mediated by blaTEM-1 was the major drug resistance mechanism.The result of MLST showed that CC155(ST-155),CC11(ST-103)and CC107(ST-1002)were the main subtypes.CONCLUSIONS The β-lactamase-producing non-typeable Hin strains are dominant among the Hin strains isolated from the adults and children with CAP in this area.The isolation rate of the strains is high among the children than among the adults.The strains are highly prevalent in autumn and winter,and ST-155 is the predominant clone type.The strains are highly resistant to ampicillin,which should be attached great importance to.
