Effects of three types of disinfection methods on decolonization of gram-negative bacteria in water sinks of neonatal wards
10.11816/cn.ni.2025-251255
- VernacularTitle:三种消毒方式对新生儿病室水槽革兰阴性菌去定植效果评价
- Author:
Peiyang FAN
1
;
Weilong ZHOU
;
Juan LI
Author Information
1. 四川大学华西第二医院/华西妇产儿童医院医院感染管理部,四川成都 610041;四川大学出生缺陷与相关妇儿疾病教育部重点实验室,四川成都 610041
- Publication Type:Journal Article
- Keywords:
Neonatal ward;
Water sink;
Gram-negative bacteria;
Decolonization;
Disinfection effect;
Chlorine-con-taining disinfectant
- From:
Chinese Journal of Nosocomiology
2025;35(21):3334-3338
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To quantitatively evaluate the effect on decolonization of gram-negative bacteria(GNB)and carbapenem-resistant gram-negative bacteria(CRGNB)in water sinks of neonatal wards and compare the decoloni-zation effects among the 500 mg/L,1000 mg/L of chlorine-containing disinfectants and 3％hydrogen peroxide so-lution.METHODS A total of 15 water sinks were chosen from West China Second University Hospital of Sichuan University as the research subjects,which were randomly divided into the 500 mg/L chlorine-containing disinfect-ant group,the 1000 mg/L chlorine-containing disinfectant group and the 3％hydrogen peroxide solution group.The bacterial colony counts of GNB and CRGNB as well as microbial diversity at the drain outlets were ob-served and compared before the intervention and after the disinfectants were continuously poured for 7 days.RESULTS There were GNB and CRGNB colonized at the drain outlets of water sinks in the neonatal wards under the mode of regular rinsing with 500 mg/L of chlorine-containing disinfectant,with Pseudomonadaceae dominant.There were no significant differences in the bacterial colony counts of GNB/CRGNB,Alpha diversity(Shannon index)and Beta diversity(Bray-Curtis distance)among the three types of disinfectants after the inter-vention for 7 days.CONCLUSIONS There are still large quantity of GNB and CRGNB colonized at the drain outlets of water sinks of neonatal wards after daily conventional treatment with 500 mg/L of chlorine-containing disinfect-ant.Neither the disinfectant with the concentration of chlorine increasing to 1000 mg/L nor 3％hydrogen peroxide solution can remarkably reduce the colonization or alter the microbial diversity.The optimal disinfection strategy should be formulated based on compound disinfectant,physical intervention and project reconstruction.
