Research of determining VK2 by LC-MS/MS method in detecting serum samples of women with postmenopausal osteoporosis
10.3969/j.issn.1672-8270.2025.08.020
- VernacularTitle:液相色谱-串联质谱法测定维生素K2方法学的建立及其对绝经后骨质疏松症妇女血清样本检测的研究
- Author:
Yu LUO
1
;
Jun WU
1
;
Chunyan LI
1
Author Information
1. 首都医科大学附属北京积水潭医院检验科 北京 100035
- Publication Type:Journal Article
- Keywords:
Liquid chromatography-tandem mass spectrometry(LC-MS/MS);
Vitamin K;
MK-4;
MK-7;
Osteoporosis
- From:
China Medical Equipment
2025;22(8):106-110,124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a determination method of liquid chromatography-tandem mass spectrometry(LC-MS/MS)for VK2 in serum,and to investigate the relationship between postmenopausal osteoporotic women and healthy women in MK-4 and MK-7 of VK2 supplements.Methods:Serum samples were collected from 126 women who admitted to Beijing Jishuitan Hospital of Capital Medical University.The women with postmenopausal osteoporosis were included in the osteoporosis group(72 cases),and the subjects without osteoporosis who received examination were included in the healthy control group(54 cases).LC-MS/MS was used to determine the levels of MK-4 and MK-7 in VK2,and the accuracy and precision were calculated.The differences in MK-4 and MK-7 between postmenopausal osteoporotic women and women who did not suffer osteoporosis were compared and analyzed.Results:In the chromatograms of standards and serum specimens detected by HPLC-MS/MS,the recoveries of different concentrations were between 101%-107%,and the precisions all met the requirements of quantitative detection(<20%).The average level of serum MK-7 of osteoporosis group was(0.48±0.12)ng/mL,which was significantly higher than(0.24±0.10)ng/ml of the healthy control group,and the difference was significant(t=11.917,P<0.05).The average level(0.07±0.02)ng/mL of serum MK-4 of the healthy control group was significantly higher than(0.04±0.01)ng/mL of osteoporosis group,and the difference was significant(t=-11.021,P<0.05).Conclusion:The LC-MS/MS method of determining VK2 that is established by this study has a series of advantages,such low cost,strong selectivity and high sensitivity,which is suitable to conventional detection for serum MK-4 and MK-7 levels,and it can simultaneously detect multiple-analytes with different concentration in serum,while it would not affect the levels of any other proteins.
