Analysis of chemical constituents of Dendrobium huoshanense flowers based on LC-MS and GC-MS
10.3969/j.issn.1001-1528.2025.11.019
- VernacularTitle:基于LC-MS和GC-MS的霍山石斛花化学成分分析
- Author:
Le-yuan JI
1
;
Qi-yan LIN
;
Jin-xiang WU
;
Qian WANG
;
Bang-xing HAN
;
Ye-cai WANG
;
Dong LIU
Author Information
1. 安徽中医药大学,安徽 合肥 230012;安徽省大别山特色生物资源保护与利用重点实验室,安徽六安 237012
- Publication Type:Journal Article
- Keywords:
Dendrobium huoshanense C.Z.Tang & S.J.Cheng;
flowers;
constituents analysis;
volatile oil;
LC-MS;
GC-MS
- From:
Chinese Traditional Patent Medicine
2025;47(11):3660-3670
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish LC-MS and GC-MS method and analyze the chemical constituents of Dendrobium huoshanense C.Z.Tang & S.J.Cheng flowers.METHODS LC-MS was performed on a Zorbax Eclipse C18 column(2.1 mm×100 mm,1.8 μm),with the mobile phase comprising of water(containing 0.1％formic acid)-acetonitrile flowing at 0.3 mL/min,and electrospray ionization was operated in both positive and negative ion modes.The GC-MS employed headspace solid-phase microextraction for sample preparation,and the analysis was performed on an HP-5MS column(30 m×0.25 mm,0.25 μm),with the following temperature program:initial temperature 50 ℃(held for 2 min),increased at 5 ℃/min to 180 ℃(held for 5 min),then raised at 10 ℃/min to 250 ℃(held for 5 min),and electron impact ion source was employed.RESULTS A total of 62 compounds were identified by LC-MS,including 35 flavonoids,4 coumarins,6 alkaloids,6 terpenoids,3 amino acids,2 polyphenols,2 ketones and 4 others.A total of 101 volatile components were identified by GC-MS,including ketones,aldehydes,alcohols,esters,ethers,and acid.CONCLUSION This method can comprehensively analyze the chemical constituents of D.huoshanense flowers,and provide a scientific basis for elucidating its pharmacodynamic material basis.
